{"measurement_id":3410,"protein_id":59,"doi":"10.1016/s0167-4838(01)00204-7","year":2001,"authors":"Jae Kwang Song; Joon Shick Rhee","title":"Enhancement of stability and activity of phospholipase A1 in organic solvents by directed evolution","journal":"Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology","enzyme_name":"Phospholipase A1 from Serratia sp. MK1","enzyme_species":"Serratia sp. MK1","ec_number":"3.1.1.32","database_id":"UniProt: Q9X9D8","source":"Recombinant, host bacterium Escherichia coli XL1-Blue","sequence":"MSMSLSFTSAIAPAAIQPPMVRTQPEPLSSSQPVEASATKAPVATLSQNSLNAQSLLNTLVSEISAAAPAAANQGVTRGQQPQKGDYTLALLAKDVYSTGSQGVEGFNRLSADALLGAGIDPASLQDAASGFQAGIYTDNQQYVLAFAGTNDMRDWLSNVRQATGYDDVQYNQAVSLAKSAKAAFGDALVIAGHSLGGGLAATAALATGTVAVTFNAAGVSDYTLNRMGIDPAAAKQDAQAGGIRRYSEQYDMLTGTQESTSLIPDAIGHKITLANNDTLSGIDDWRPSKHLDRSLTAHGIDKVISSMAEQKPWEAMANA","is_extremophile":"No","mutation":"WT","property":"Stability - Incubation","procedure":"Activity after incubation (10 hours at 37\u00b0C), measured by measuring product concentration by TLC-FID in aqueous phase","solvent_name":"Dimethyl Sulfoxide","solvent_concentration":"40","solvent_concentration_unit":"% v/v","aqueous_control":"100","wt_control":"NaN","measured_value":"3","units":"%","assay_solution":"Incubation: 50 mM Tris-HCl, 10 mM CaCl2, 450 microM CHAPS, Assay: 50 mM Tris-HCl, 10 mM CaCl2, 450 microM CHAPS","ph":"Incubation: 9, Assay: 9","temperature":"Incubation: 37\u00b0C, Assay: 40\u00b0C","substrates":"1,2-dimyristoyl-sn-glycero-3-phosphocholine","substrate_concentrations":"2 mL of 20 microM","products":"2-myristoyl-lysophosphatidylcholine, Myristic acid","cofactor":"NaN","shaking":"Yes, \"thorough shaking\" assay","extraction_method":"Digital","data_type":"Continuous","comments":"Non-incubated control (in %), assay in aqueous phase"}