Carboxylesterase from Sulfolobus solfataricus P1

Enzyme Description

Species
Sulfolobus solfataricus P1 β†—
Extremophile
Yes "thermoacidophilic" organism
EC Number
3.1.1.1 β†—

Sequence

Length: 305 amino acids
MPLDPTIKRLLESGFVIPIGKVPVDEVRKVFRQISSAAPKMDVGNIEDIKIPGTETMIPARVYYPKTKGPYGILVYLHGGGFVIGDIESYDPVCRAITNACNCVVASIDYRLAPENKFPSAVVDSVDATKWIYENAESLEGKQGVAVGGDSAGGNLSAVVSILTKGKINLKHQVLIYPATGADYTSRSMVEYYDGYFLTREQIEWFGSQYLRSYMDLLDIKFSPILIQDLSGLPPALIITAEYDPLRDQGEAYANKLLMAGIPVTSVRFNNVIHGFVSFFPLIPQGMDAIGLIGSTLTVAFYNSF
Y Park et al. (2006) β€” A carboxylesterase from the thermoacidophilic archaeon Sulfolobus solfataricus P1; purification, characterization, and expression
Biochimica et Biophysica Acta (BBA) - General Subjects  Β· doi:10.1016/j.bbagen.2006.01.009 β†—  Β· Stability - Incubation
24 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Purified, archaeon Sulfolobus solfataricus P1

Experimental Data (24 measurements)

24 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 90 % v/v 100 27 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 90 % v/v 100 34 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 70Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 90 % v/v 100 20 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 70Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 90 % v/v 100 9 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 70Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 90 % v/v 100 3 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 70Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 40 % v/v 100 87 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 70Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 40 % v/v 100 58 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 70Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 40 % v/v 100 83 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 70Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 40 % v/v 100 77 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 70Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 90 % v/v 100 45 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 90 % v/v 100 43 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 90 % v/v 100 32 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (0 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 40 % v/v 100 88 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 40 % v/v 100 91 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 40 % v/v 100 61 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 40 % v/v 100 90 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 40 % v/v 100 86 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (0 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 90 % v/v 100 93 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (0 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 90 % v/v 100 76 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (0 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 90 % v/v 100 82 % Direct Continuous Incubation: 100 mM sodium phosphate, Assay: 100 mM sodium phosphate buffer with sodium taurocholate (2 mg/ml) and gum arabic (1 mg/ml) as emulsifiers Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 0.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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