Ene-reductase XenB from Pseudomonas fluorescens

Enzyme Description

Species
Pseudomonas fluorescens β†—
Extremophile
No
EC Number
1.6.99.1 β†—

Sequence

Length: 349 amino acids
MATIFDPIKLGDLELSNRIIMAPLTRCRADEGRVPNALMAEYYVQRASAGLILSEATSVTPMGVGYPDTPGIWSNDQVRGWTNITKAVHAAGGKIVLQLWHVGRISHPLYLNGEAPVAPSAIQPKGHVSLVRPLADYPTPRALETAEIAEIVEAYRTGAENAKAAGFDGVEIHGANGYLLDQFLQSSTNQRTDNYGGSLENRARLLLEVTDAAIDVWGAGRVGVHLAPRADSHDMGDDNLAETFTYVARELGKRGIAFICSREKEGADSLGPQLKEAFGGAYIANERFTKDSANAWLAEGKADAVAFGVPFIANPDLPARLKADAPLNEPRPELFYGKGPVGYIDYPTL
Frieda A. Sorgenfrei et al. (2024) β€” Solvent concentration at 50% protein unfolding may reform enzyme stability ranking and process window identification
Nature Communications  Β· doi:10.1038/s41467-024-49774-0 β†—  Β· Activity - Classical Stability - Tm
60 measurements ▼
Database ID
UniProt: Q9RPM1 β†—
Sequence Annotation
Explicit - Provided UniProt Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (60 measurements)

60 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 20 % v/v 100.0 78.2 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 56.6 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15 % v/v 100.0 48.7 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100.0 37.5 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25 % v/v 100.0 27.0 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100.0 13.8 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 5 % v/v 100.0 87.3 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 10 % v/v 100.0 88.5 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 15 % v/v 100.0 84.8 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 20 % v/v 100.0 81.2 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 25 % v/v 100.0 72.1 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 30 % v/v 100.0 49.1 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 5 % v/v 100.0 100.0 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 10 % v/v 100.0 96.5 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 15 % v/v 100.0 90.8 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100.0 75.7 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 25 % v/v 100.0 53.5 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 43.7 % Direct Continuous 50 mM sodium phosphate 7.4 25Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 5 % v/v 100.0 98.6 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 10 % v/v 100.0 99.3 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Tm

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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