Mutant laccase MtLT2 from Myceliophthora thermophila

Enzyme Description

Species

Extremophile

EC Number

1.10.3.2 ↗

Sequence

Length: 559 amino acids

QQICNTPSNRACWTDGYDINTDYEVDSPDTGVVRPYTLTLTEVDNWTGPDGVVKEKVMLVNNSIIGPTIFADWGDTIQVTVINNLGTNGTSIHWHGLHQKGTNLHDGVNGITECPIPPKGGRKVYRFKAQQYGTSWYHSHFSAQYGNGVVGAIQINGPASLPYDTDLGVFPISDYYYSSADELVELTKNSGAPFSDNVLFNGTAKHPETGEGEYANVTLTPGRRHRLRLINTSVENHFQVSLVNHTMTIIAADMVPVNAMTVDSLFLGVGQRYDVVIEASRTPGNYWFNVTFGGGLLCGGSRSPYPAAIFHYAGAPGGPPTDEGKAPVDHNCLDLPNLKPVVARDVPLSGLAKRPDNTLDVTLDTMGTPLFVWKVNGSAINIDWGRPVVDYVLTQNTSFPPGHNIVEVNGADQWSYWLIENDPGAPFTLPHPMHLHGHDFYVLGRSPDEPPASKERHVFDPARDAGLLSGANPVRRDVTMLPAFGWVVLAFRADNPGAWLFHCHIAWHVSGGLGVVYLERADDLRGAVSDADADDFDRLCADWRRYWPTNPNPKSDSGL

Miren Zumárraga et al. (2007) — In Vitro Evolution of a Fungal Laccase in High Concentrations of Organic Cosolvents

Chemistry & Biology · doi:10.1016/j.chembiol.2007.08.010 ↗ · Activity - Classical Stability - Incubation

110 measurements ▼

Database ID

—

Sequence Annotation

Inferred - from protein name (11 mutations compared to PDB 6F5K, article denomination : MtLT2)

Protein Source

Recombinant, host yeast Saccharomyces cerevisiae

Experimental Data (110 measurements)

110 measurements — page 2 of 6

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
E182K/L429V (7H9)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Acetonitrile	30 % v/v	4767	149	974	mmol product/(mmol laccase * min)	Direct	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in mmol product/(mmol laccase * min))
S280N/L429V/N552H (2B10)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	50 % v/v	5841	58	435	mmol product/(mmol laccase * min)	Direct	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in mmol product/(mmol laccase * min))
S280N/L429V/N552H (2B10)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Acetonitrile	30 % v/v	5841	149	600	mmol product/(mmol laccase * min)	Direct	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in mmol product/(mmol laccase * min))
E182K/L429V/N552H (3D1)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	50 % v/v	4085	58	524	mmol product/(mmol laccase * min)	Direct	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in mmol product/(mmol laccase * min))
E182K/L429V/N552H (3D1)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Acetonitrile	30 % v/v	4085	149	780	mmol product/(mmol laccase * min)	Direct	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in mmol product/(mmol laccase * min))
E182K/S280N/L429V/N552H (R2)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	50 % v/v	5619	58	1106	mmol product/(mmol laccase * min)	Direct	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in mmol product/(mmol laccase * min))
E182K/S280N/L429V/N552H (R2)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Acetonitrile	30 % v/v	5619	149	1876	mmol product/(mmol laccase * min)	Direct	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in mmol product/(mmol laccase * min))
L429V (6C9)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	10 % v/v	100	49	61	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
L429V (6C9)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	20 % v/v	100	25	38	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
L429V (6C9)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	30 % v/v	100	14	23	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
L429V (6C9)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	40 % v/v	100	6	11	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
L429V (6C9)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	50 % v/v	100	3	5	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
L429V (6C9)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	60 % v/v	100	0	2	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
S280N/L429V/N552H (2B10)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	10 % v/v	100	49	68	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
S280N/L429V/N552H (2B10)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	20 % v/v	100	25	49	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
S280N/L429V/N552H (2B10)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	30 % v/v	100	14	28	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
S280N/L429V/N552H (2B10)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	40 % v/v	100	6	13	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
S280N/L429V/N552H (2B10)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	50 % v/v	100	3	7	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
S280N/L429V/N552H (2B10)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	60 % v/v	100	0	2	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
S280N/L429V/N552H (2B10)	Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent	Ethanol	10 % v/v	100	49	58	%	Digital	Continuous	100 mM sodium acetate buffer	4.5	Room temperature	3 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

1703

—

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

1703

—

149

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

—

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

—

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

—

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

—

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

—

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

60 % v/v

100

—

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100

—

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100

—

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100

—

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

40 % v/v

100

—

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

1703

—

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

—

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Non-incubated control (in %) measured in the presence of organic solvent, assay measured in the presence of organic solvent | Clear about assay and control conditions (both in the presence of organic solvent)

Stability - Incubation

Acetonitrile

30 % v/v

100

—

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

3579

198

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

3579

149

449

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

2048

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

2048

149

314

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

E182K/L429V (7H9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

4767

222

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

E182K/L429V (7H9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

4767

149

974

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

5841

435

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

5841

149

600

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

4085

524

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

4085

149

780

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

5619

1106

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

5619

149

1876

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

60 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

60 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

60 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

60 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

60 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

60 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

L429V (6C9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S280N/L429V/N552H (2B10)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V (7H9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V (7H9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V (7H9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V (7H9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

S179P/L429V (5B9)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/L429V/N552H (3D1)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

E182K/S280N/L429V/N552H (R2)

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Acetonitrile

40 % v/v

100

Digital

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in %)

L429V

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

3423

205

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

E182K

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

2298

142

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

N552H

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

2167

203

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

S280N

Activity - Classical

Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 418 nm) in the presence of organic solvent

Ethanol

50 % v/v

1999

188

mmol product/(mmol laccase * min)

Direct

Continuous

100 mM sodium acetate buffer

4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

Classical aqueous control (in mmol product/(mmol laccase * min))

L429V (6C9)

Stability - Incubation

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

L429V (6C9)

Stability - Incubation

Acetonitrile

30 % v/v

100

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

S179P/L429V (5B9)

Stability - Incubation

Ethanol

50 % v/v

100

125

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

S179P/L429V (5B9)

Stability - Incubation

Acetonitrile

30 % v/v

100

155

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/L429V (7H9)

Stability - Incubation

Ethanol

50 % v/v

100

110

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/L429V (7H9)

Stability - Incubation

Acetonitrile

30 % v/v

100

138

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

S280N/L429V/N552H (2B10)

Stability - Incubation

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

S280N/L429V/N552H (2B10)

Stability - Incubation

Acetonitrile

30 % v/v

100

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/L429V/N552H (3D1)

Stability - Incubation

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/L429V/N552H (3D1)

Stability - Incubation

Acetonitrile

30 % v/v

100

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/S280N/L429V/N552H (R2)

Stability - Incubation

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/S280N/L429V/N552H (R2)

Stability - Incubation

Acetonitrile

30 % v/v

100

Direct

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/S280N/L429V/N552H (R2)

Stability - Incubation

Dimethylformamide (DMF)

60 % v/v

100

—

Digital

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/S280N/L429V/N552H (R2)

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

—

100

Digital

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/S280N/L429V/N552H (R2)

Stability - Incubation

Dimethylacetamide

60 % v/v

100

—

Digital

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/S280N/L429V/N552H (R2)

Stability - Incubation

Ethanol

60 % v/v

100

—

Digital

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/S280N/L429V/N552H (R2)

Stability - Incubation

Acetone

60 % v/v

100

—

100

Digital

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/S280N/L429V/N552H (R2)

Stability - Incubation

Methanol

60 % v/v

100

—

100

Digital

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

E182K/S280N/L429V/N552H (R2)

Stability - Incubation

Acetonitrile

60 % v/v

100

—

Digital

Continuous

Incubation: 100 mM phosphat buffer, Assay: 20 mM sodium acetate buffer

Incubation: 6, Assay: 4.5

Room temperature

3 mM ABTS

ABTS radical cation

—

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Mutations in this dataset (11)

WT S179P/L429V (5B9) E182K E182K/L429V (7H9) E182K/L429V/N552H (3D1) E182K/S280N/L429V/N552H (R2) S280N S280N/L429V/N552H (2B10) L429V L429V (6C9) N552H

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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Mutant laccase MtLT2 from Myceliophthora thermophila

Enzyme Description

Sequence

Experimental Data (110 measurements)

Visualization : Activity — Classical

Mutation Effect

Visualization : Stability — Incubation

Mutation Effect

Structure

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