Xanthine oxidase from Bos taurus (Bovine)

Enzyme Description

Species

Extremophile

EC Number

1.17.3.2 ↗

Sequence

Length: 1332 amino acids

MTADELVFFVNGKKVVEKNADPETTLLAYLRRKLGLRGTKLGCGEGGCGACTVMLSKYDRLQDKIIHFSANACLAPICTLHHVAVTTVEGIGSTKTRLHPVQERIAKSHGSQCGFCTPGIVMSMYTLLRNQPEPTVEEIEDAFQGNLCRCTGYRPILQGFRTFAKNGGCCGGNGNNPNCCMNQKKDHTVTLSPSLFNPEEFMPLDPTQEPIFPPELLRLKDVPPKQLRFEGERVTWIQASTLKELLDLKAQHPEAKLVVGNTEIGIEMKFKNQLFPMIICPAWIPELNAVEHGPEGISFGAACALSSVEKTLLEAVAKLPTQKTEVFRGVLEQLRWFAGKQVKSVASLGGNIITASPISDLNPVFMASGTKLTIVSRGTRRTVPMDHTFFPSYRKTLLGPEEILLSIEIPYSREDEFFSAFKQASRREDDIAKVTCGMRVLFQPGSMQVKELALCYGGMADRTISALKTTQKQLSKFWNEKLLQDVCAGLAEELSLSPDAPGGMIEFRRTLTLSFFFKFYLTVLKKLGKDSKDKCGKLDPTYTSATLLFQKDPPANIQLFQEVPNGQSKEDTVGRPLPHLAAAMQASGEAVYCDDIPRYENELFLRLVTSTRAHAKIKSIDVSEAQKVPGFVCFLSADDIPGSNETGLFNDETVFAKDTVTCVGHIIGAVVADTPEHAERAAHVVKVTYEDLPAIITIEDAIKNNSFYGSELKIEKGDLKKGFSEADNVVSGELYIGGQDHFYLETHCTIAIPKGEEGEMELFVSTQNAMKTQSFVAKMLGVPVNRILVRVKRMGGGFGGKETRSTLVSVAVALAAYKTGHPVRCMLDRNEDMLITGGRHPFLARYKVGFMKTGTIVALEVDHYSNAGNSRDLSHSIMERALFHMDNCYKIPNIRGTGRLCKTNLSSNTAFRGFGGPQALFIAENWMSEVAVTCGLPAEEVRWKNMYKEGDLTHFNQRLEGFSVPRCWDECLKSSQYYARKSEVDKFNKENCWKKRGLCIIPTKFGISFTVPFLNQAGALIHVYTDGSVLVSHGGTEMGQGLHTKMVQVASKALKIPISKIYISETSTNTVPNSSPTAASVSTDIYGQAVYEACQTILKRLEPFKKKNPDGSWEDWVMAAYQDRVSLSTTGFYRTPNLGYSFETNSGNAFHYFTYGVACSEVEIDCLTGDHKNLRTDIVMDVGSSLNPAIDIGQVEGAFVQGLGLFTLEELHYSPEGSLHTRGPSTYKIPAFGSIPTEFRVSLLRDCPNKKAIYASKAVGEPPLFLGASVFFAIKDAIRAARAQHTNNNTKELFRLDSPATPEKIRNACVDKFTTLCVTGAPGNCKPWSLRV

M. R. Rashidi et al. (2009) — Catalytic activity and stability of xanthine oxidase in aqueous-organic mixtures

Biochemistry (Moscow) · doi:10.1134/s0006297909010155 ↗ · Activity - Classical Stability - C50

153 measurements ▼

Database ID

UniProt: P80457 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Commercially purchased

Experimental Data (153 measurements)

153 measurements — page 1 of 8

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	2.5 % v/v	100.0	76.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	5 % v/v	100.0	43.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	7.5 % v/v	100.0	22.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10 % v/v	100.0	11.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	12.5 % v/v	100.0	5.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	15 % v/v	100.0	3.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	17.5 % v/v	100.0	1.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20 % v/v	100.0	0.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	22.5 % v/v	100.0	0.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	25 % v/v	100.0	0.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	27.5 % v/v	100.0	0.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	30 % v/v	100.0	0.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	2.5 % v/v	100.0	93.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5 % v/v	100.0	81.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	7.5 % v/v	100.0	72.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10 % v/v	100.0	62.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	12.5 % v/v	100.0	49.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15 % v/v	100.0	41.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	17.5 % v/v	100.0	30.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20 % v/v	100.0	24.0	%	Digital	Continuous	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

2.5 % v/v

100.0

76.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

5 % v/v

100.0

43.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

7.5 % v/v

100.0

22.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100.0

11.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

12.5 % v/v

100.0

5.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

15 % v/v

100.0

3.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

17.5 % v/v

100.0

1.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

22.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

25 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

27.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

30 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

2.5 % v/v

100.0

93.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

5 % v/v

100.0

81.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

7.5 % v/v

100.0

72.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

10 % v/v

100.0

62.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

12.5 % v/v

100.0

49.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

15 % v/v

100.0

41.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

17.5 % v/v

100.0

30.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

20 % v/v

100.0

24.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

22.5 % v/v

100.0

20.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

25 % v/v

100.0

12.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

27.5 % v/v

100.0

9.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Dimethylformamide (DMF)

30 % v/v

100.0

5.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

2.5 % v/v

100.0

90.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

5 % v/v

100.0

68.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

7.5 % v/v

100.0

49.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

10 % v/v

100.0

39.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

12.5 % v/v

100.0

19.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

15 % v/v

100.0

9.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

17.5 % v/v

100.0

6.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

20 % v/v

100.0

3.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

22.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

25 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

27.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1-Propanol

30 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

2.5 % v/v

100.0

98.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

5 % v/v

100.0

81.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

7.5 % v/v

100.0

60.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100.0

49.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

12.5 % v/v

100.0

43.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

15 % v/v

100.0

35.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

17.5 % v/v

100.0

28.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100.0

18.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

22.5 % v/v

100.0

13.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

25 % v/v

100.0

9.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

27.5 % v/v

100.0

6.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Isopropanol

30 % v/v

100.0

3.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

2.5 % v/v

100.0

81.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

5 % v/v

100.0

77.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

7.5 % v/v

100.0

67.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

10 % v/v

100.0

60.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

12.5 % v/v

100.0

56.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

15 % v/v

100.0

49.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

17.5 % v/v

100.0

44.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

20 % v/v

100.0

34.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

22.5 % v/v

100.0

33.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

25 % v/v

100.0

26.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

27.5 % v/v

100.0

18.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

1,4-Dioxane

30 % v/v

100.0

9.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

2.5 % v/v

100.0

60.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

5 % v/v

100.0

21.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

7.5 % v/v

100.0

7.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

10 % v/v

100.0

2.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

12.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

15 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

17.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

20 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

22.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

25 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

27.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Pyridine

30 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

2.5 % v/v

100.0

14.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

5 % v/v

100.0

4.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

7.5 % v/v

100.0

2.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

10 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

12.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

15 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

17.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

20 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

22.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

25 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

27.5 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Methanol

30 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

2.5 % v/v

100.0

92.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

5 % v/v

100.0

75.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

7.5 % v/v

100.0

70.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100.0

54.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

12.5 % v/v

100.0

45.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

15 % v/v

100.0

32.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

17.5 % v/v

100.0

26.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100.0

14.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

22.5 % v/v

100.0

9.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

25 % v/v

100.0

4.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

27.5 % v/v

100.0

2.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100.0

0.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

2.5 % v/v

100.0

98.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

5 % v/v

100.0

87.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

7.5 % v/v

100.0

80.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

10 % v/v

100.0

66.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

12.5 % v/v

100.0

61.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

15 % v/v

100.0

50.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

17.5 % v/v

100.0

37.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

20 % v/v

100.0

30.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

22.5 % v/v

100.0

16.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

25 % v/v

100.0

11.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

27.5 % v/v

100.0

8.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Ethanol

30 % v/v

100.0

1.0

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Classical aqueous control (in %)

Stability - C50

Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

—

7.5

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

27°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

—

4.9

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

32°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

—

4.3

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

—

3.1

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

42°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

—

2.2

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

50°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Dimethylformamide (DMF)

—

12.6

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

27°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Dimethylformamide (DMF)

—

15.2

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

32°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Dimethylformamide (DMF)

—

12.6

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Dimethylformamide (DMF)

—

2.6

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

42°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Dimethylformamide (DMF)

—

12.4

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

50°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

1-Propanol

—

11.2

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

27°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

1-Propanol

—

8.5

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

32°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

1-Propanol

—

7.3

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

1-Propanol

—

5.8

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

42°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

1-Propanol

—

6.9

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

50°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Isopropanol

—

13.6

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

27°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Isopropanol

—

10.6

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

32°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Isopropanol

—

9.3

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Isopropanol

—

8.5

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

42°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Isopropanol

—

10.7

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

50°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

1,4-Dioxane

—

15.0

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

27°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

1,4-Dioxane

—

17.9

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

32°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

1,4-Dioxane

—

15.0

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

1,4-Dioxane

—

6.1

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

42°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

1,4-Dioxane

—

14.9

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

50°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Pyridine

—

3.7

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

27°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Pyridine

—

3.4

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

32°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Pyridine

—

3.3

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Pyridine

—

3.0

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

42°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Pyridine

—

3.1

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

50°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Methanol

—

1.8

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

27°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Methanol

—

1.4

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

32°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Methanol

—

1.3

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Methanol

—

1.8

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

42°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Methanol

—

1.2

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

50°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Acetonitrile

—

11.2

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

27°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Acetonitrile

—

11.2

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

32°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Acetonitrile

—

10.8

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Acetonitrile

—

8.1

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

42°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Acetonitrile

—

8.0

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

50°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Ethanol

—

18.1

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

27°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Ethanol

—

15.8

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

32°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Ethanol

—

14.9

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

37°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Ethanol

—

13.8

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

42°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

Stability - C50

Ethanol

—

8.1

% (v/v)

Digital

Continuous

67 mM phosphate buffer, 0.1 M EDTA

50°C

20 µM Xanthine

Uric Acid

—

Not applicable control (in % (v/v))

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — C₅₀

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Structure

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Xanthine oxidase from Bos taurus (Bovine)

Enzyme Description

Sequence

Experimental Data (153 measurements)

Visualization : Activity — Classical

Visualization : Stability — C50

Structure

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Visualization : Stability — C₅₀