Ene-reductase YqjM from Bacillus subtilis

Enzyme Description

Species
Bacillus subtilis β†—
Extremophile
No
EC Number
1.6.99.1 β†—

Sequence

Length: 338 amino acids
MARKLFTPITIKDMTLKNRIVMSPMCMYSSHEKDGKLTPFHMAHYISRAIGQVGLIIVEASAVNPQGRITDQDLGIWSDEHIEGFAKLTEQVKEQGSKIGIQLAHAGRKAELEGDIFAPSAIAFDEQSATPVEMSAEKVKETVQEFKQAAARAKEAGFDVIEIHAAHGYLIHEFLSPLSNHRTDEYGGSPENRYRFLREIIDEVKQVWDGPLFVRVSASDYTDKGLDIADHIGFAKWMKEQGVDLIDCSSGALVHADINVFPGYQVSFAEKIREQADMATGAVGMITDGSMAEEILQNGRADLIFIGRELLRDPFFARTAAKQLNTEIPAPVQYERGW
Frieda A. Sorgenfrei et al. (2024) β€” Solvent concentration at 50% protein unfolding may reform enzyme stability ranking and process window identification
Nature Communications  Β· doi:10.1038/s41467-024-49774-0 β†—  Β· Activity - Classical Stability - Tm
60 measurements ▼
Database ID
UniProt: P54550 β†—
Sequence Annotation
Explicit - Provided UniProt Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (60 measurements)

60 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 15 % v/v 100.0 32.7 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 20 % v/v 100.0 22.4 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 25 % v/v 100.0 28.6 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 30 % v/v 100.0 12.2 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent 1-Propanol 5 % v/v 100.0 50.0 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent 1-Propanol 10 % v/v 100.0 34.0 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent 1-Propanol 15 % v/v 100.0 24.0 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent 1-Propanol 20 % v/v 100.0 10.0 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent 1-Propanol 25 % v/v 100.0 0.0 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent 1-Propanol 30 % v/v 100.0 0.0 % Direct Continuous 50 mM sodium phosphate 7.4 Room temperature 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Methanol 20 % v/v 51.0 39.33 Β°C Direct Continuous 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 51.0 48.0 Β°C Direct Continuous 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15 % v/v 51.0 46.67 Β°C Direct Continuous 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 51.0 46.0 Β°C Direct Continuous 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25 % v/v 51.0 45.0 Β°C Direct Continuous 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 51.0 44.0 Β°C Direct Continuous 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Ethanol 5 % v/v 51.0 45.0 Β°C Direct Continuous 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Ethanol 10 % v/v 51.0 42.33 Β°C Direct Continuous 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Ethanol 15 % v/v 51.0 38.33 Β°C Direct Continuous 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Ethanol 20 % v/v 51.0 35.0 Β°C Direct Continuous 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Tm

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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