Transaminase ArR from Arthrobacter sp.

Enzyme Description

Species
Arthrobacter sp. β†—
Extremophile
No
EC Number
2.6.1.- β†—

Sequence

Length: 330 amino acids
MAFSADTSEIVYTHDTGLDYITYSDYELDPANPLAGGAAWIEGAFVPPSEARISIFDQGYLHSDVTYTVFHVWNGNAFRLDDHIERLFSNAESMRIIPPLTQDEVKEIALELVAKTELREAFVSVSITRGYSSTPGERDITKHRPQVYMYAVPYQWIVPFDRIRDGVHAMVAQSVRRTPRSSIDPQVKNFQWGDLIRAVQETHDRGFEAPLLLDGDGLLAEGSGFNVVVIKDGVVRSPGRAALPGITRKTVLEIAESLGHEAILADITLAELLDADEVLGCTTAGGVWPFVSVDGNPISDGVPGPITQSIIRRYWELNVESSSLLTPVQY
Frieda A. Sorgenfrei et al. (2024) β€” Solvent concentration at 50% protein unfolding may reform enzyme stability ranking and process window identification
Nature Communications  Β· doi:10.1038/s41467-024-49774-0 β†—  Β· Stability - C50
9 measurements ▼
Database ID
UniProt: F7J696 β†—
Sequence Annotation
Explicit - Provided PDB Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (9 measurements)

9 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 19 % (v/v) Direct Continuous 50 mM sodium phosphate 7.4 25Β°C β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 18 % (v/v) Direct Continuous 50 mM sodium phosphate 7.4 30Β°C β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 18 % (v/v) Direct Continuous 50 mM sodium phosphate 7.4 35Β°C β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 19 % (v/v) Direct Continuous 50 mM sodium phosphate 7.4 40Β°C β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 20 % (v/v) Direct Continuous 50 mM sodium phosphate 7.4 45Β°C β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 18 % (v/v) Direct Continuous 50 mM sodium phosphate 7.4 50Β°C β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 14 % (v/v) Direct Continuous 50 mM sodium phosphate 7.4 60Β°C β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 11 % (v/v) Direct Continuous 50 mM sodium phosphate 7.4 70Β°C β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 7 % (v/v) Direct Continuous 50 mM sodium phosphate 7.4 80Β°C β€” β€” β€” β€” Not applicable control (in % (v/v))

Visualization : Stability β€” C50

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Structure

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