Laccase from Phoma sp. UHH 5-1-03 (DSM 22425)
Enzyme Description
Sequence
MKSIFTLGVALLGLSSHVQGAAVKVERMEMTRIQPRNDLVERQSAACGSNGPNSRHCWTPGFTSSTDMYNSWPNTGQIVSYNLRIENTTCNPDGNGSRVCMLINGRMPGPTIVANWGDTIRVTVRNLLQHNGTSIHWHGFRMLNKVIQDGTNGVTECALAPGDIKTYEFQATEYGTTWYHSHFSHQYGDGVVGTVQINGPATANYDVDLGVMPMTDWYYITAFQAARRAATGGGGPPLPDNVLINGTSKNAAGGGAWNKVSIQSGKRYRLRLVNTAVDTNFMVNLDNHNFQVIATDFVPVEPYNTSYLQIGIGQRYDVIFTANQTAGNYWFRAVANSNCQSRAAREGRAVFTYQGQTVADPTSNAQGNPPTGCTDPVTTPKIVKTVPSSTFAAQAKSLPVAFGPVAVQNNLVLWTINGTSQVVDPGNPTIKYVAENNNNFPQALNVVEVSSTSATTWTYWVIQQAANAPPIAHPIHLHGHDSYILGQGTGQFNVTTHFSQLRFNNPPRRDVAQLQGGGWLVLAYPTDNPGAWLMHCHIAFHVGMGLSIQFLERKQSINLPAPGSQWFQNCNNWAAYKAGNTDVYPQDDSGLRKRWPALGAENSMAEW
C. Junghanns et al. (2009)
β Biochemical and molecular genetic characterisation of a novel laccase produced by the aquatic ascomycete Phoma sp. UHH 5-1-03
Applied Microbiology and Biotechnology
Β· doi:10.1007/s00253-009-2028-2 β
Β· Stability - C50 Stability - Complete Inhibition
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Database ID
UniProt: B3TLA6 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Purified, fungus Phoma sp. UHH 5-1-03 (DSM 22425)
Experimental Data (12 measurements)
12 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - C50 | Volume fraction of organic solvent for which half of the activity is lost at 25Β°C, measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent | Acetone | β | β | 31.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent for which half of the activity is lost at 25Β°C, measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent | Acetonitrile | β | β | 4.5 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent for which half of the activity is lost at 25Β°C, measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | β | β | 40.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent for which half of the activity is lost at 25Β°C, measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent | Ethanol | β | β | 52.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent for which half of the activity is lost at 25Β°C, measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent | Isopropanol | β | β | 60.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent for which half of the activity is lost at 25Β°C, measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent | Methanol | β | β | 52.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - Complete Inhibition | Volume of organic solvent for complete inhibition (with activities measured by absorbance spectrophotometry) | Acetone | β | β | 80.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - Complete Inhibition | Volume of organic solvent for complete inhibition (with activities measured by absorbance spectrophotometry) | Acetonitrile | β | β | >80.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - Complete Inhibition | Volume of organic solvent for complete inhibition (with activities measured by absorbance spectrophotometry) | Dimethyl Sulfoxide (DMSO) | β | β | 60.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - Complete Inhibition | Volume of organic solvent for complete inhibition (with activities measured by absorbance spectrophotometry) | Ethanol | β | β | >80.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - Complete Inhibition | Volume of organic solvent for complete inhibition (with activities measured by absorbance spectrophotometry) | Isopropanol | β | β | >80.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
| Stability - Complete Inhibition | Volume of organic solvent for complete inhibition (with activities measured by absorbance spectrophotometry) | Methanol | β | β | 80.0 | % (v/v) | Direct | Continuous | 0.1 M citrate buffer | 4 | 25Β°C | 3 mM ABTS | ABTS radical cation reaction products | β | β | Not applicable control (in % (v/v)) |
Visualization : Stability β Complete Inhibition
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β C50
One bar per measurement. Colour = solvent, shade = temperature. Hover for details.