Purified hydroxynitrile lyase (gene PeHNL) from Passiflora edulis (passion fruit)

Enzyme Description

Species

Extremophile

EC Number

4.1.2.10 ↗

Sequence

Length: 147 amino acids

MRNPGASFLIKHFLVLSLFLCAGTAHNPPEIVRHIVFNRYKSQLSQKQIDQIIADYGNLQNIAPEMKEWKWGTDLGPAVEDRADGFTHAYESTFHSVADFLNFFYSPPALEFAKEFFPACEKIVVLNYIINETFPYTWALPNKYVVT

Techawaree Ueatrongchit et al. (2010) — Hydroxynitrile lyase from Passiflora edulis: Purification, characteristics and application in asymmetric synthesis of (R)-mandelonitrile

Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2010.02.008 ↗ · Activity - Classical Stability - Incubation Selectivity - Enantioselectivity

32 measurements ▼

Database ID

UniProt: A0A1L7NZN4 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, eukaryote Passiflora edulis (passion fruit)

Experimental Data (32 measurements)

32 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Butyl Ether	80 % (v/v)	30.0	0.74	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Butyl Ether	80 % (v/v)	30.0	10.8	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Butyl Ether	90 % (v/v)	30.0	10.2	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Butyl Ether	95 % (v/v)	30.0	7.9	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Butyl Ether	70 % (v/v)	30.0	11.5	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Butyl Ether	60 % (v/v)	30.0	12.7	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Butyl Ether	50 % (v/v)	30.0	13.4	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Butyl Ether	40 % (v/v)	30.0	11.5	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	n-Hexane	80 % (v/v)	30.0	3.5	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Diisopropyl Ether	80 % (v/v)	30.0	1.16	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	tert-Butyl Methyl Ether (MTBE)	80 % (v/v)	30.0	0.23	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Diethyl Ether	80 % (v/v)	30.0	0.42	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Activity - Classical	Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent	Ethyl Acetate	80 % (v/v)	30.0	0.0	µM/hour	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in microM/hour)
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent	Butyl Ether	80 % (v/v)	59.0	99.0	%	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in %)
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent	Butyl Ether	90 % (v/v)	59.0	100.0	%	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in %)
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent	Butyl Ether	95 % (v/v)	59.0	100.0	%	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in %)
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent	Butyl Ether	80 % (v/v)	59.0	99.3	%	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in %)
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent	Butyl Ether	70 % (v/v)	59.0	99.0	%	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in %)
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent	Butyl Ether	60 % (v/v)	59.0	98.4	%	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in %)
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent	Butyl Ether	50 % (v/v)	59.0	98.0	%	Digital	Continuous	400 mM citrate-phosphate buffer	4	10°C	500 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	1500 rpm	Classical aqueous control (in %)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Butyl Ether

80 % (v/v)

30.0

0.74

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Butyl Ether

80 % (v/v)

30.0

10.8

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Butyl Ether

90 % (v/v)

30.0

10.2

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Butyl Ether

95 % (v/v)

30.0

7.9

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Butyl Ether

70 % (v/v)

30.0

11.5

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Butyl Ether

60 % (v/v)

30.0

12.7

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Butyl Ether

50 % (v/v)

30.0

13.4

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Butyl Ether

40 % (v/v)

30.0

11.5

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

n-Hexane

80 % (v/v)

30.0

3.5

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Diisopropyl Ether

80 % (v/v)

30.0

1.16

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

tert-Butyl Methyl Ether (MTBE)

80 % (v/v)

30.0

0.23

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Diethyl Ether

80 % (v/v)

30.0

0.42

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Activity - Classical

Initial reaction rate (in µM/hour) measured by HPLC in the presence of organic solvent

Ethyl Acetate

80 % (v/v)

30.0

0.0

µM/hour

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in µM/hour)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Butyl Ether

80 % (v/v)

59.0

99.0

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Butyl Ether

90 % (v/v)

59.0

100.0

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Butyl Ether

95 % (v/v)

59.0

100.0

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Butyl Ether

80 % (v/v)

59.0

99.3

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Butyl Ether

70 % (v/v)

59.0

99.0

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Butyl Ether

60 % (v/v)

59.0

98.4

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Butyl Ether

50 % (v/v)

59.0

98.0

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Butyl Ether

40 % (v/v)

59.0

97.5

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

n-Hexane

80 % (v/v)

59.0

69.0

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Diisopropyl Ether

80 % (v/v)

59.0

100.0

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

tert-Butyl Methyl Ether (MTBE)

80 % (v/v)

59.0

100.0

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Diethyl Ether

80 % (v/v)

59.0

100.0

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) measured by HPLC in the presence of organic solvent

Ethyl Acetate

80 % (v/v)

59.0

100.0

Digital

Continuous

400 mM citrate-phosphate buffer

10°C

500 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

1500 rpm

Classical aqueous control (in %)

—

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by chromatography in aqueous phase

Ethyl Acetate

50 % (v/v)

100.0

42.0

Digital

Continuous

400 mM citrate-phosphate buffer, 400 mM citrate-phosphate buffer assay

Incubation: 4, Assay: 4

Incubation: 10°C, Assay: 10°C

Acetone cyanohydrin, Benzaldehyde

(R)-Mandelonitrile

—

Incubation: 1500 rpm

Water-incubated control (in %), assay and control in aqueous phase

—

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by chromatography in aqueous phase

n-Hexane

50 % (v/v)

100.0

88.0

Digital

Continuous

400 mM citrate-phosphate buffer, 400 mM citrate-phosphate buffer assay

Incubation: 4, Assay: 4

Incubation: 10°C, Assay: 10°C

Acetone cyanohydrin, Benzaldehyde

(R)-Mandelonitrile

—

Incubation: 1500 rpm

Water-incubated control (in %), assay and control in aqueous phase

—

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by chromatography in aqueous phase

Butyl Ether

50 % (v/v)

100.0

92.0

Digital

Continuous

400 mM citrate-phosphate buffer, 400 mM citrate-phosphate buffer assay

Incubation: 4, Assay: 4

Incubation: 10°C, Assay: 10°C

Acetone cyanohydrin, Benzaldehyde

(R)-Mandelonitrile

—

Incubation: 1500 rpm

Water-incubated control (in %), assay and control in aqueous phase

—

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by chromatography in aqueous phase

Diisopropyl Ether

50 % (v/v)

100.0

86.0

Digital

Continuous

400 mM citrate-phosphate buffer, 400 mM citrate-phosphate buffer assay

Incubation: 4, Assay: 4

Incubation: 10°C, Assay: 10°C

Acetone cyanohydrin, Benzaldehyde

(R)-Mandelonitrile

—

Incubation: 1500 rpm

Water-incubated control (in %), assay and control in aqueous phase

—

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by chromatography in aqueous phase

tert-Butyl Methyl Ether (MTBE)

50 % (v/v)

100.0

Digital

Continuous

400 mM citrate-phosphate buffer, 400 mM citrate-phosphate buffer assay

Incubation: 4, Assay: 4

Incubation: 10°C, Assay: 10°C

Acetone cyanohydrin, Benzaldehyde

(R)-Mandelonitrile

—

Incubation: 1500 rpm

Water-incubated control (in %), assay and control in aqueous phase

—

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by chromatography in aqueous phase

Diethyl Ether

50 % (v/v)

100.0

70.0

Digital

Continuous

400 mM citrate-phosphate buffer, 400 mM citrate-phosphate buffer assay

Incubation: 4, Assay: 4

Incubation: 10°C, Assay: 10°C

Acetone cyanohydrin, Benzaldehyde

(R)-Mandelonitrile

—

Incubation: 1500 rpm

Water-incubated control (in %), assay and control in aqueous phase

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Visualization : Selectivity — Enantioselectivity

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Aem Nuylert et al. (2017) — Effect of Glycosylation on the Biocatalytic Properties of Hydroxynitrile Lyase from the Passion Fruit, Passiflora edulis : A Comparison of Natural and Recombinant Enzymes

ChemBioChem · doi:10.1002/cbic.201600447 ↗ · Stability - Incubation Selectivity - Enantioselectivity

12 measurements ▼

Database ID

UniProt: A0A1L7NZN4 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Purified, eukaryote Passiflora edulis (passion fruit)

Experimental Data (12 measurements)

12 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC	Ethyl Acetate	50 % (v/v)	50.4	96.4	%	Digital	Continuous	400 mM citrate buffer	4	10°C	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC	Diethyl Ether	50 % (v/v)	50.4	95.2	%	Digital	Continuous	400 mM citrate buffer	4	10°C	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC	tert-Butyl Methyl Ether (MTBE)	50 % (v/v)	50.4	95.9	%	Digital	Continuous	400 mM citrate buffer	4	10°C	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC	Diisopropyl Ether	50 % (v/v)	50.4	96.6	%	Digital	Continuous	400 mM citrate buffer	4	10°C	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC	Butyl Ether	50 % (v/v)	50.4	94.2	%	Digital	Continuous	400 mM citrate buffer	4	10°C	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Selectivity - Enantioselectivity	Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC	n-Hexane	50 % (v/v)	50.4	47.2	%	Digital	Continuous	400 mM citrate buffer	4	10°C	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Stability - Incubation	Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent	Ethyl Acetate	50 % (v/v)	88.7	68.3	%	Digital	Continuous	Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer	Incubation: 4, Assay: 4	?	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Stability - Incubation	Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent	Diethyl Ether	50 % (v/v)	88.7	89.5	%	Digital	Continuous	Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer	Incubation: 4, Assay: 4	?	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Stability - Incubation	Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent	tert-Butyl Methyl Ether (MTBE)	50 % (v/v)	88.7	81.3	%	Digital	Continuous	Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer	Incubation: 4, Assay: 4	?	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Stability - Incubation	Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent	Diisopropyl Ether	50 % (v/v)	88.7	81.3	%	Digital	Continuous	Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer	Incubation: 4, Assay: 4	?	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Stability - Incubation	Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent	Butyl Ether	50 % (v/v)	88.7	70.0	%	Digital	Continuous	Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer	Incubation: 4, Assay: 4	?	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN
Stability - Incubation	Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent	n-Hexane	50 % (v/v)	88.7	70.0	%	Digital	Continuous	Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer	Incubation: 4, Assay: 4	?	900 mM Acetone cyanohydrin, 250 mM Benzaldehyde	(R)-Mandelonitrile	—	—	Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value \| Acetone cyanohydrin forms HCN

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC

Ethyl Acetate

50 % (v/v)

50.4

96.4

Digital

Continuous

400 mM citrate buffer

10°C

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC

Diethyl Ether

50 % (v/v)

50.4

95.2

Digital

Continuous

400 mM citrate buffer

10°C

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC

tert-Butyl Methyl Ether (MTBE)

50 % (v/v)

50.4

95.9

Digital

Continuous

400 mM citrate buffer

10°C

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC

Diisopropyl Ether

50 % (v/v)

50.4

96.6

Digital

Continuous

400 mM citrate buffer

10°C

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC

Butyl Ether

50 % (v/v)

50.4

94.2

Digital

Continuous

400 mM citrate buffer

10°C

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Selectivity - Enantioselectivity

Enantiomeric excess (toward R form) after 12 hours incubation at 10°C in the presence of organic solvent, measured by HPLC

n-Hexane

50 % (v/v)

50.4

47.2

Digital

Continuous

400 mM citrate buffer

10°C

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Classical aqueous control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Stability - Incubation

Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent

Ethyl Acetate

50 % (v/v)

88.7

68.3

Digital

Continuous

Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer

Incubation: 4, Assay: 4

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Stability - Incubation

Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent

Diethyl Ether

50 % (v/v)

88.7

89.5

Digital

Continuous

Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer

Incubation: 4, Assay: 4

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Stability - Incubation

Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent

tert-Butyl Methyl Ether (MTBE)

50 % (v/v)

88.7

81.3

Digital

Continuous

Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer

Incubation: 4, Assay: 4

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Stability - Incubation

Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent

Diisopropyl Ether

50 % (v/v)

88.7

81.3

Digital

Continuous

Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer

Incubation: 4, Assay: 4

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Stability - Incubation

Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent

Butyl Ether

50 % (v/v)

88.7

70.0

Digital

Continuous

Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer

Incubation: 4, Assay: 4

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Stability - Incubation

Activity after incubation (12 hours at 10°C), measured by HPLC in presence of organic solvent

n-Hexane

50 % (v/v)

88.7

70.0

Digital

Continuous

Incubation: 400 mM citrate buffer, Assay: 400 mM citrate buffer

Incubation: 4, Assay: 4

900 mM Acetone cyanohydrin, 250 mM Benzaldehyde

(R)-Mandelonitrile

—

Water-incubated control (in %), with activity measured in the presence of organic solvent before and after incubation for determining 100% value | Acetone cyanohydrin forms HCN

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Visualization : Selectivity — Enantioselectivity

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Structure

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Purified hydroxynitrile lyase (gene PeHNL) from Passiflora edulis (passion fruit)

Enzyme Description

Sequence

Experimental Data (32 measurements)

Visualization : Activity — Classical

Visualization : Stability — Incubation

Visualization : Selectivity — Enantioselectivity

Experimental Data (12 measurements)

Visualization : Stability — Incubation

Visualization : Selectivity — Enantioselectivity

Structure

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