Lipase E0YJE9 from a biomass metagenomic library

Enzyme Description

Species
Na (biomass metagenomic library)
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 289 amino acids
MPMKINRYAIGSAALAAGLLMSSAAFAVNPGGGGSGFASLYNRAESDGRCSVTRLNVGSSTFYIPRDPSNPNARFNVLVWGNGTGGNSLTYATLLESVASHCIAVAAANTANSGTGVEMQEAYNSLRSRYGNILGSKVCTAGHSQGGGGSFNAANRIGANCVIPVQPDTRFTTRIYSPLASHVEVITLWGQIDTLAPASGNRSNVERASTILTQVETSGEGHFAPVSGRGGKIGTMFRMANIAQLSNDPATAQEFRRAFWGPTTDYTASTSHPDISEVRRNAAAQATTP
AmΓ©lie CΓ΄tΓ© et al. (2010) β€” Expression and characterization of a novel heterologous moderately thermostable lipase derived from metagenomics in Streptomyces lividans
Journal of Industrial Microbiology & Biotechnology  Β· doi:10.1007/s10295-010-0735-4 β†—  Β· Stability - Incubation
12 measurements ▼
Database ID
UniProt: E0YJE9 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Streptomyces lividans strain 10-164

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10 % v/v 100 102 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100 100 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 10 % v/v 100 91 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 10 % v/v 100 103 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 10 % v/v 100 94 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 10 % v/v 100 113 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 30 % v/v 100 121 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30 % v/v 100 105 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 30 % v/v 100 116 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 30 % v/v 100 121 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 30 % v/v 100 112 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 30 % v/v 100 106 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.05% CaCl2 Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 60Β°C ? mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), assay and control in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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