DNA polymerase eta from Homo sapiens

Enzyme Description

Species
Homo sapiens (human) β†—
Extremophile
No
EC Number
2.7.7.7 β†—

Sequence

Length: 713 amino acids
MATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLPQGPTTAEETVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVIKNCNTSGIQTEWSPPLTMLFLCATKFSASAPSSSTDITSFLSSDPSSLPKVPVTSSEAKTQGSGPAVTATKKATTSLESFFQKAAERQKVKEASLSSLTAPTQAPMSNSPSKPSLPFQTSQSTGTEPFFKQKSLLLKQKQLNNSSVSSPQQNPWSNCKALPNSLPTEYPGCVPVCEGVSKLEESSKATPAEMDLAHNSQSMHASSASKSVLEVTQKATPNPSLLAAEDQVPCEKCGSLVPVWDMPEHMDYHFALELQKSFLQPHSSNPQVVSAVSHQGKRNPKSPLACTNKRPRPEGMQTLESFFKPLTH
Sayo Kashiwagi et al. (2010) β€” Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana
Journal of Nucleic Acids  Β· doi:10.4061/2010/701472 β†—  Β· Activity - Classical
9 measurements ▼
Database ID
UniProt: Q9Y253 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli Rosetta2 (DE3)

Experimental Data (9 measurements)

9 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry Dimethyl Sulfoxide (DMSO) 20 % v/v 100 81 % Digital Continuous 40 mM Tris-HCl buffer, 1mM MgClβ‚‚, 10 mM DTT, 60 mM KCl, 2.5% glycerol 8 37Β°C 40 nM 32P-labeled primer-templates, 100 mM dNTPs DNA double strand incorporating dCTPs β€” β€” Classical aqueous control (in %)
Activity - Classical Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry Ethanol 20 % v/v 100 79 % Digital Continuous 40 mM Tris-HCl buffer, 1mM MgClβ‚‚, 10 mM DTT, 60 mM KCl, 2.5% glycerol 8 37Β°C 40 nM 32P-labeled primer-templates, 100 mM dNTPs DNA double strand incorporating dCTPs β€” β€” Classical aqueous control (in %)
Activity - Classical Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry Isopropanol 20 % v/v 100 26 % Digital Continuous 40 mM Tris-HCl buffer, 1mM MgClβ‚‚, 10 mM DTT, 60 mM KCl, 2.5% glycerol 8 37Β°C 40 nM 32P-labeled primer-templates, 100 mM dNTPs DNA double strand incorporating dCTPs β€” β€” Classical aqueous control (in %)
Activity - Classical Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry Dimethyl Sulfoxide (DMSO) 40 % v/v 100 16 % Digital Continuous 40 mM Tris-HCl buffer, 1mM MgClβ‚‚, 10 mM DTT, 60 mM KCl, 2.5% glycerol 8 37Β°C 40 nM 32P-labeled primer-templates, 100 mM dNTPs DNA double strand incorporating dCTPs β€” β€” Classical aqueous control (in %)
Activity - Classical Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry Dimethyl Sulfoxide (DMSO) 5 % v/v 100 89 % Digital Continuous 40 mM Tris-HCl buffer, 1mM MgClβ‚‚, 10 mM DTT, 60 mM KCl, 2.5% glycerol 8 37Β°C 40 nM 32P-labeled primer-templates, 100 mM dNTPs DNA double strand incorporating dCTPs β€” β€” Classical aqueous control (in %)
Activity - Classical Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry Dimethyl Sulfoxide (DMSO) 10 % v/v 100 62 % Digital Continuous 40 mM Tris-HCl buffer, 1mM MgClβ‚‚, 10 mM DTT, 60 mM KCl, 2.5% glycerol 8 37Β°C 40 nM 32P-labeled primer-templates, 100 mM dNTPs DNA double strand incorporating dCTPs β€” β€” Classical aqueous control (in %)
Activity - Classical Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry Dimethyl Sulfoxide (DMSO) 20 % v/v 100 49 % Digital Continuous 40 mM Tris-HCl buffer, 1mM MgClβ‚‚, 10 mM DTT, 60 mM KCl, 2.5% glycerol 8 37Β°C 40 nM 32P-labeled primer-templates, 100 mM dNTPs DNA double strand incorporating dCTPs β€” β€” Classical aqueous control (in %)
Activity - Classical Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry Dimethyl Sulfoxide (DMSO) 30 % v/v 100 21 % Digital Continuous 40 mM Tris-HCl buffer, 1mM MgClβ‚‚, 10 mM DTT, 60 mM KCl, 2.5% glycerol 8 37Β°C 40 nM 32P-labeled primer-templates, 100 mM dNTPs DNA double strand incorporating dCTPs β€” β€” Classical aqueous control (in %)
Activity - Classical Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry Dimethyl Sulfoxide (DMSO) 40 % v/v 100 5 % Digital Continuous 40 mM Tris-HCl buffer, 1mM MgClβ‚‚, 10 mM DTT, 60 mM KCl, 2.5% glycerol 8 37Β°C 40 nM 32P-labeled primer-templates, 100 mM dNTPs DNA double strand incorporating dCTPs β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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