Lipase from Geobacillus sp. ARM

Enzyme Description

Species
Geobacillus sp. ARM β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 418 amino acids
MMKCCRRVALVLLGLWLVFCLSVPGGQAEAAASRANDAPIVLLHGFTGWGREEMFGFKYWGGVRGDIEQLLNAQGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRVHIIAHSQGGQTARMLVSLLENGSKEEREYAKAHNVSLSPLFEGGHNFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLKAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDQYFKRLKQSPVWTSADTARYDLSVPGAEALNQWVQASPNTYYLSFATERTYRGALTGNYYPELGMNVFSAAVCAPFLGSYRNAALGIDDRWLENDGIVNTFSMNGPKRGSTDRIVPYDGTLKKGVWNDMGTYNVDHLEVIGVDPNPLFDIHAFYLRLAEQLASLQP
Afshin Ebrahimpour et al. (2011) β€” High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM
Bioresource Technology  Β· doi:10.1016/j.biortech.2011.03.083 β†—  Β· Activity + Stability - Incubation
27 measurements ▼
Database ID
UniProt: A0A0D8BQX7 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli TOP10

Experimental Data (27 measurements)

27 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Toluene 30 % v/v 100 165 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100 62 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent 2,3-Butanediol 30 % v/v 100 100 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Methanol 30 % v/v 100 107 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100 1 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Ethanol 30 % v/v 100 116 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Acetone 30 % v/v 100 2 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Isopropanol 30 % v/v 100 3 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent 1-Propanol 30 % v/v 100 1 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Isobutanol 30 % v/v 100 4 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent 1-Butanol 30 % v/v 100 1 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Benzyl Alcohol 30 % v/v 100 0 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Isoamyl Alcohol 30 % v/v 100 21 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Benzene 30 % v/v 100 142 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent n-Hexadecane 30 % v/v 100 77 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent 1-Octanol 30 % v/v 100 137 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent o-Xylene 30 % v/v 100 136 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent Ethylbenzene 30 % v/v 100 77 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent n-Hexane 30 % v/v 100 139 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent 1-Decanol 30 % v/v 100 86 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl Incubation: 7, Assay: 8 Incubation: 30Β°C, Assay: 65Β°C emulsion : 50% v/v Olive oil free fatty acids , glycerol β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
β€Ή Prev
1 2
Next β€Ί

Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*