Esterase (gene EstA) with mesophilic expression from Sulfolobus islandicus

Enzyme Description

Species

Sulfolobus islandicus ↗

Extremophile

Yes thermophilic organism

EC Number

3.1.1.1 ↗

Sequence

Length: 305 amino acids

MPLDPRIKELLESGFIVPIGKASVDEVRKIFRQLASAAPKVEVGKVEDIKIPGSEANINARVYLPKANGPYGVLIYLHGGGFVIGDVESYDPLCRAITNACNCVVVSVDYRLAPEYKFPSAVIDSFDATNWVYNNLDKFDGKMGVAIAGDSAGGNLAAVVALLSKGKLNLKYQILIYPAVGFDSVSRSMIEYSDGFFLTREHIEWFGSQYLRSPADLLDFRFSPILAQDLSGLPPALIITAEYDPLRDQGEAYANRLLQAGVPVTSVRFNNVIHGFLSFFPLIEQGRDAISLIGSVLRRTFYDKS

Yuxia Mei et al. (2011) — Exceptional thermal stability and organic solvent tolerance of an esterase expressed from a thermophilic host

Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3504-z ↗ · Stability - Incubation

22 measurements ▼

Database ID

UniProt: F0NDQ1 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethanol	30 % v/v	100.0	41.4	%	Direct	Continuous	Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)	Incubation: 7, Assay: 8	Incubation: 70°C, Assay: 65°C	8 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), residual activity measured in aqueous phase \| Uncertainty about whether assay was really in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Methanol	60 % v/v	100.0	23.1	%	Direct	Continuous	Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)	Incubation: 7, Assay: 8	Incubation: 70°C, Assay: 65°C	8 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), residual activity measured in aqueous phase \| Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Dimethyl Sulfoxide (DMSO)

60 % v/v

100.0

12.3

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Chloroform

60 % v/v

100.0

0.0

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Chloroform

30 % v/v

100.0

0.0

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

n-Hexane

60 % v/v

100.0

0.0

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

n-Hexane

30 % v/v

100.0

10.2

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Isoamyl Alcohol

60 % v/v

100.0

0.0

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Isoamyl Alcohol

30 % v/v

100.0

7.9

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

1-Butanol

60 % v/v

100.0

6.4

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

1-Butanol

30 % v/v

100.0

17.9

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Acetonitrile

60 % v/v

100.0

4.3

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Acetonitrile

30 % v/v

100.0

39.7

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Methanol

30 % v/v

100.0

61.3

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

73.0

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Isopropanol

60 % v/v

100.0

19.1

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Isopropanol

30 % v/v

100.0

30.2

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

1-Propanol

60 % v/v

100.0

9.2

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

1-Propanol

30 % v/v

100.0

31.6

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Acetone

60 % v/v

100.0

13.7

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Acetone

30 % v/v

100.0

63.0

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Ethanol

60 % v/v

100.0

17.5

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Ethanol

30 % v/v

100.0

41.4

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

Stability - Incubation

Activity after incubation (30 minutes at 70°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Methanol

60 % v/v

100.0

23.1

Direct

Continuous

Incubation: 50 mM phosphate buffer, 150 mM NaCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic (according to reference)

Incubation: 7, Assay: 8

Incubation: 70°C, Assay: 65°C

8 mM p-Nitrophenyl hexanoate

Hexanoic Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity measured in aqueous phase | Uncertainty about whether assay was really in aqueous phase

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Esterase (gene EstA) with mesophilic expression from Sulfolobus islandicus

Enzyme Description

Sequence

Experimental Data (22 measurements)

Visualization : Stability — Incubation

Structure

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