Protease (gene sapB) from Bacillus pumilus CBS

Enzyme Description

Species

Extremophile

EC Number

3.4.- ↗

Sequence

Length: 275 amino acids

AQTVPYGIPQIKAPAVHAQGYKGANVKVAVLDTGIHAAHPDLNVAGGASFVPSEPNATQDFQSHGTHVAGTIAALDNTIGVLGVAPSASLYAVKVLDRNGDGQYSWIISGIEWAVANNMDVINMSLGGPNGSTALKNAVDTANNRGVVVVAAAGNSGSTGSTSTVGYPAKYDSTIAVANVNSSNVRNSSSSAGPELDVSAPGTSILSTVPSSGYTSYTGTSMASPHVAGAAALILSKYPNLSTTQVRQRLENTATPLGNSFYYGKGLINVQAASN

Nadia Zaraî Jaouadi et al. (2012) — The overexpression of the SAPB of Bacillus pumilus CBS and mutated sapB-L31I/T33S/N99Y alkaline proteases in Bacillus subtilis DB430: New attractive properties for the mutant enzyme

Bioresource Technology · doi:10.1016/j.biortech.2011.11.115 ↗ · Stability - Incubation

56 measurements ▼

Database ID

UniProt: A8FBN9 ↗

Sequence Annotation

Inferred - from protein name (106 first residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Bacillus subtilis DB430

Experimental Data (56 measurements)

56 measurements — page 3 of 3

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (240 hours at 40°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Dimethylformamide (DMF)	60 % v/v	100	105	155	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 40°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (240 hours at 40°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60 % v/v	100	64	90	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 40°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60 % v/v	100	140	160	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Dimethylformamide (DMF)	60 % v/v	100	190	225	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Methanol	60 % v/v	100	100	100	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Ethanol	60 % v/v	100	70	80	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Acetonitrile	60 % v/v	100	0	0	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Isopropanol	60 % v/v	100	105	125	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Ethyl Acetate	60 % v/v	100	70	91	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	1-Butanol	60 % v/v	100	40	50	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Toluene	60 % v/v	100	114	121	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	Cyclohexane	60 % v/v	100	131	148	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	n-Hexane	60 % v/v	100	150	180	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	n-Octane	60 % v/v	100	124	141	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	n-Decane	60 % v/v	100	110	131	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
L31I/T33S/N99Y	Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase	n-Hexadecane	60 % v/v	100	112	135	%	Direct	Continuous	Incubation: ?, Assay: 100 mM Gly-NaOH	Incubation: ?, Assay: 10	Incubation: 50°C, Assay: 70°C	0.01 Casein	free amino acids , Peptides	—	Incubation: 180 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase

n-Decane

60 % v/v

100

—

110

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

n-Hexadecane

60 % v/v

100

—

112

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Activity after incubation (240 hours at 40°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reactant and free tyrosines reaction product absorbance measurement, 440 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Dimethylformamide (DMF)

60 % v/v

100

—

105

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Methanol

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Ethanol

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Acetonitrile

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Isopropanol

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Ethyl Acetate

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

1-Butanol

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Toluene

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Cyclohexane

60 % v/v

100

—

109

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

n-Hexane

60 % v/v

100

—

117

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

n-Octane

60 % v/v

100

—

106

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

n-Decane

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

—

140

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

n-Hexadecane

60 % v/v

100

—

101

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

n-Octane

60 % v/v

100

—

124

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

n-Hexane

60 % v/v

100

—

150

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Cyclohexane

60 % v/v

100

—

131

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Toluene

60 % v/v

100

—

114

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

1-Butanol

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Ethyl Acetate

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Isopropanol

60 % v/v

100

—

105

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Acetonitrile

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Ethanol

60 % v/v

100

—

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Methanol

60 % v/v

100

—

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Stability - Incubation

Dimethylformamide (DMF)

60 % v/v

100

—

190

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 65°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

n-Hexadecane

60 % v/v

100

101

111

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

n-Decane

60 % v/v

100

107

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

n-Octane

60 % v/v

100

106

122

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

n-Hexane

60 % v/v

100

117

135

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Cyclohexane

60 % v/v

100

109

129

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Toluene

60 % v/v

100

102

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

1-Butanol

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Ethyl Acetate

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Isopropanol

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Acetonitrile

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Ethanol

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Methanol

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Dimethylformamide (DMF)

60 % v/v

100

105

155

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 40°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

140

160

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Dimethylformamide (DMF)

60 % v/v

100

190

225

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Methanol

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Ethanol

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Acetonitrile

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Isopropanol

60 % v/v

100

105

125

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Ethyl Acetate

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

1-Butanol

60 % v/v

100

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Toluene

60 % v/v

100

114

121

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

Cyclohexane

60 % v/v

100

131

148

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

n-Hexane

60 % v/v

100

150

180

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

n-Octane

60 % v/v

100

124

141

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

n-Decane

60 % v/v

100

110

131

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

L31I/T33S/N99Y

Stability - Incubation

n-Hexadecane

60 % v/v

100

112

135

Direct

Continuous

Incubation: ?, Assay: 100 mM Gly-NaOH

Incubation: ?, Assay: 10

Incubation: 50°C, Assay: 70°C

0.01 Casein

free amino acids, Peptides

—

Incubation: 180 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

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Mutations in this dataset (2)

WT L31I/T33S/N99Y

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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Protease (gene sapB) from Bacillus pumilus CBS

Enzyme Description

Sequence

Experimental Data (56 measurements)

Visualization : Stability — Incubation

Mutation Effect

Structure

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