Endo-Ξ²-1,4-mannanase from Bacillus subtilis G1

Enzyme Description

Species
Bacillus subtilis G1 β†—
Extremophile
No
EC Number
3.2.1.78 β†—

Sequence

Length: 364 amino acids
MGSCIVKNIRSLAHYILLRLLFSKTNEAHTVSPVNPNAQQTTKAVMNWLAHLPNRTENRVLSGAFGGYSHDTFSMAEADRIRSATGQSPAIYGCDYARGWLETAKIEDSIDVSCNGDLISYWKNGGIPQISLHLANPAFQSGHFKTPITNDQYKKILDSSTAEGKRLNAMLSKIADGLQELENQGVPVLFRPLHEMNGEWFWWGLTSYNQKDNERISLYKQLYKKIYHYMTDTRGLDHLIWVYSPDANRDFKTDFYPGASYVDIVGLDAYFQDAYSINGYDQLTALNKPFAFTEVGPQPANGSFDYSLFINAIKQKYPKTIYFLAWNDEWSPAVNKGASALYHDSWTLNKGEIWNGDSLTPIVE
Thi Thu Hang Vu et al. (2012) β€” Cloning, High-Level Expression, Purification, and Properties of a Novel Endo-Ξ²-1,4-Mannanase from Bacillus subtilis G1 in Pichia pastoris
Journal of Microbiology and Biotechnology  Β· doi:10.4014/jmb.1106.06052 β†—  Β· Stability - Incubation
15 measurements ▼
Database ID
UniProt: Q2LGZ6 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host yeast Pichia pastoris GS115

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Methanol 10 % v/v 100 78 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethanol 10 % v/v 100 89 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Isopropanol 10 % v/v 100 100 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase 1-Butanol 10 % v/v 100 65 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetone 10 % v/v 100 85 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Methanol 20 % v/v 100 20 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethanol 20 % v/v 100 71 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Isopropanol 20 % v/v 100 106 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase 1-Butanol 20 % v/v 100 1 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetone 20 % v/v 100 88 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Methanol 30 % v/v 100 3 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethanol 30 % v/v 100 88 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Isopropanol 30 % v/v 100 103 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase 1-Butanol 30 % v/v 100 0 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (DNS method) Acetone 30 % v/v 100 46 % Direct Continuous Incubation: ?, Assay: 20 mM potassium buffer Incubation: ?, Assay: 7 Incubation: 37Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Non-incubated control (in %), reaction in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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