Azoreductase from Shewanella oneidensis MR-1

Enzyme Description

Species
Shewanella oneidensis MR-1 β†—
Extremophile
No but "organic-solvent tolerant" enzyme
EC Number
1.7.1.17 β†—

Sequence

Length: 198 amino acids
MSKVLVLKSSILGGYSQSALLVDYLIGKWEKQGATITVRDLAGKDVLPMVDGEIASGLRGGAELTARQQEMLDLSNALVEELKANDTIVITAPMYNFNIPTQLKNWIDFVARAGVTFTYTENGPKGLVEGKRAVLITTRGGAHKDGPTDHMVPFLKTFLGFIGITDVDVVYAEALNMGPEANQKGISEAKASIDKLAV
Yuyi Yang et al. (2012) β€” Characterization of an efficient catalytic and organic solvent-tolerant azoreductase toward methyl red from Shewanella oneidensis MR-1
Environmental Science and Pollution Research  Β· doi:10.1007/s11356-012-1221-5 β†—  Β· Stability - Incubation
48 measurements ▼
Database ID
UniProt: Q8E990 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (48 measurements)

48 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Methanol 80 % v/v 100.0 0.0 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Ethanol 80 % v/v 100.0 0.0 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Isopropanol 80 % v/v 100.0 0.0 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase 1-Propanol 80 % v/v 100.0 0.0 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 30 % v/v 100.0 99.32 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Acetone 10 % v/v 100.0 98.06 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Methanol 10 % v/v 100.0 109.11 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Ethanol 10 % v/v 100.0 108.32 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Isopropanol 10 % v/v 100.0 109.04 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase 1-Propanol 10 % v/v 100.0 90.39 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 20 % v/v 100.0 104.91 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Acetone 20 % v/v 100.0 105.8 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Methanol 20 % v/v 100.0 102.49 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Ethanol 20 % v/v 100.0 107.83 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Isopropanol 20 % v/v 100.0 103.88 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase 1-Propanol 20 % v/v 100.0 1.85 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 95.08 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Acetone 30 % v/v 100.0 104.3 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Methanol 30 % v/v 100.0 103.33 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Ethanol 30 % v/v 100.0 20.73 % Direct Continuous Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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