Aldehyde oxidase 1 from Oryctolagus cuniculus (rabbit)

Enzyme Description

Species
Oryctolagus cuniculus (rabbit) β†—
Extremophile
No
EC Number
1.2.3.1 β†—

Sequence

Length: 1334 amino acids
MEPAPELLFYVNGRKVVEKQVDPETMLLPYLRKKLRLTGTKYGCGGGGCGACTVMISRYNRVTKKIRHYPVNACLTPICSLYGAAVTTVEGIGSTTTRLHPVQERIAKFHGTQCGFCTPGMVMSMYALLRNHPEPTLDQLADALGGNLCRCTGYRPIIEAYKTFCKTSDCCQNKENGFCCLDQGINGLPEVEEENQTRPNLFSEEEYLPLDPTQELIFPPELMTMAEKQPQRTRVFSGERMMWISPVTLKALLEAKSTYPQAPVVMGNTSVGPGVKFKGIFHPVIISPDSIEELNVVSHTHSGLTLGAGLSLAQVKDILADVVQKVPEENAQTYRALLKHLGTLAGSQIRNMASLGGHIISRHLDSDLNPLLAVGNCTLNVLSKEGERQIPLDEQFLSRCPEADLKPQEILASVHIPYSRKWEFVLAFRQAQRKQNALAIVNSGMRVFFGEGDGIIRELAISYGGVGPTIICAKNSCQKLIGRSWNEEMLDTACRLILDEVSLPGSAPGGKVEFKRTLIISFLFKFYLEVSQILKRMAPGLSPHLADKYESALQDLHARYSWSTLKDQDVDARQLSQDPIGHPVMHLSGVKHATGEAIYLDDMPAVDQELFMAFVTSPRAHAKIVSTDLLEALSLPGVVDIVTAEHLQDGNTFYTEKLLAADEVLCVGQLVCAVIAESEVQAKQAAKQVKIVYEDLEPVILSIEEAIEQKSFFEPERKLEYGNVDEAFKVVDQILEGEIHMGGQEHFYMETQSVLVVPKGEDQEMDVYASTQFPKYIQDMVAAVLKLPVNKVMCHVKRVGGAFGGKVFKASIMAAIAAFAANKHGRAVRCILERGEDMLITGGRHPYLGKYKAGFMNDGRIVALDVEHYSNGGCSLDESLLVIEMGLLKMENAYKFPNLRCRGWACRTNLPSNTAFRGFGFPQAGLITECCITEVAAKCGLSPEKVRAINFYKEIDQTPYKQEINAKNLTQCWNECLAKSSYFQRKVAVEKFNAENYWKQRGLAIIPFKYPRGLGSVAYGQAAALVHVYLDGSVLVTHGGIEMGQGVHTKMIQVVSRELKMPMSNVHLRGTSTETVPNTNASGGSVVADLNGLAVKDACQTLLKRLEPIINKNPQGTWKEWAQAAFDKSISLSATGYFRGYDSNIDWDKGEGHPFEYFVYGAACSEVEIDCLTGDHKTIRTDIVMDVGYSINPALDIGQVEGAFIQGMGLYTIEELHYSPQGILYSRGPNQYKIPAICDIPAELNVTFLPPSEKSNTLYSSKGLGESGVFMGCSVFFAIREAVCAARQARGLSAPWKLSSPLTPEKIRMACEDKFTKMIPRDKPGSYVPWNVPV
Mohammad-Reza Rashidi et al. (2013) β€” Aldehyde Oxidase Activity and Stability in Water-Miscible Organic Solvents
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-012-0036-7 β†—  Β· Activity - Classical Activity - Michaelis-Menten Stability - C50 Stability - Incubation
243 measurements ▼
Database ID
UniProt: P80456 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, eukaryote Oryctolagus cuniculus (rabbit)

Experimental Data (243 measurements)

243 measurements β€” page 5 of 13
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Methanol 20 % v/v 100.0 0.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Methanol 25 % v/v 100.0 0.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 0.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Methanol 35 % v/v 100.0 0.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 0.5 % v/v 100.0 95.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 1 % v/v 100.0 88.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 1.5 % v/v 100.0 83.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 2 % v/v 100.0 79.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 2.5 % v/v 100.0 72.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 5 % v/v 100.0 63.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100.0 41.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 15 % v/v 100.0 17.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100.0 0.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 25 % v/v 100.0 0.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100.0 0.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile 35 % v/v 100.0 0.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Ethanol 0.5 % v/v 100.0 98.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Ethanol 1 % v/v 100.0 93.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Ethanol 1.5 % v/v 100.0 88.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Ethanol 2 % v/v 100.0 83.0 % Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity β€” Michaelis-Menten

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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” C50

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Structure

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