Deglycosylated Ξ²-glucuronidase from Aspergillus oryzae
Enzyme Description
Sequence
MLKPQQTTTRDLISLDGLWKFALASDDNNTQPWTSQLKTSLECPVPASYNDIFADSKIHDHVGWVYYQRDVIVPKGWSEERYLVRCEAATHHGRIYVNGNLVADHVGGYTPFEADITDLVAAGEQFRLTIAVDNELTYQTIPPGKVEILEATGKKVQTYQHDFYNYAGLARSVWLYSVPQQHIQDITVRTDVQGTTGLIDYNVVASTTQGTIQVAVIDEDGTTVATSSGSNGTIHIPSVHLWQPGAAYLYQLHASIIDSSKKTIDTYKLATGIRTVKVQGTQFLINDKPFYFTGFGKHEDTNIRGKGHDDAYMVHDFQLLHWMGANSFRTSHYPYAEEVMEYADRQGIVVIDETPAVGLAFSIGAGAQTSNPPATFSPDRINNKTREAHAQAIRELIHRDKNHPSVVMWSIANEPASNEDGAREYFAPLPKLARQLDPTRPVTFANVGLATYKADRIADLFDVLCLNRYFGWYTQTAELDEAEAALEEELRGWTEKYDKPIVMTEYGADTVAGLHSVMVTPWSEEFQVEMLDMYHRVFDRFEAMAGEQVWNFADFQTAVGVSRVDGNKKGVFTRDRKPKAAAHLLRKRWTNLHNGTAEGGKTFQ
Shuping Zou et al. (2013)
β N-glycosylation enhances functional and structural stability of recombinant Ξ²-glucuronidase expressed in Pichia pastoris
Journal of Biotechnology
Β· doi:10.1016/j.jbiotec.2012.12.015 β
Β· Activity - Classical
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Database ID
UniProt: A7XS03 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host yeast Pichia pastoris GS115
Experimental Data (12 measurements)
12 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Dimethyl Sulfoxide (DMSO) | 10 % v/v | 100.0 | 102.3 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Dimethyl Sulfoxide (DMSO) | 20 % v/v | 100.0 | 104.5 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Dimethyl Sulfoxide (DMSO) | 30 % v/v | 100.0 | 110.1 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Dimethyl Sulfoxide (DMSO) | 40 % v/v | 100.0 | 107.2 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Dimethyl Sulfoxide (DMSO) | 50 % v/v | 100.0 | 90.8 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Dimethyl Sulfoxide (DMSO) | 60 % v/v | 100.0 | 27.3 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Acetone | 10 % v/v | 100.0 | 99.2 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Acetone | 20 % v/v | 100.0 | 92.5 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Acetone | 30 % v/v | 100.0 | 74.5 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Acetone | 40 % v/v | 100.0 | 42.4 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Acetone | 50 % v/v | 100.0 | 20.4 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC (Ξ²-mono-glucuronide-glycyrrhizin production) | Acetone | 60 % v/v | 100.0 | 0.0 | % | Direct | Continuous | 100 mM sodium acetate buffer | 5 | 40Β°C | 0.0016 Glycyrrhizin | beta-mono-glucuronide-glycyrrhizin , glucuronic acid | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.