Copper-containing oxidase from Thermobifida fusca NTU10-1

Enzyme Description

Species
Thermobifida fusca NTU10-1 β†—
Extremophile
No but "moderately thermophilic" organism
EC Number
1.10.3.2 β†— (UniProt annotations in contradiction with publication)

Sequence

Length: 241 amino acids
VTGTVVELAPGIHAGFTGRAGGVSGEPYATLNLGDHVGDDPAAVAENRRRAALGFGISPDRVVWMNQVHGATAVTVTGSGQAGDVDAVVTPEAGLALAVLVADCLPLLVADAAAGVIGAAHAGRPGMAAGVVPALVAEMARHGARPERCVALLGPAICGRCYEVPRDLQDRVARTVPEARCTTAEGTPGLDIRAGVTAQLTNLGVTNITHDSRCTRESADLFSYRRDATTGRFAGYVWRVP
Cheng-Yu Chen et al. (2013) β€” A 24.7-kDa copper-containing oxidase, secreted by Thermobifida fusca, significantly increasing the xylanase/cellulase-catalyzed hydrolysis of sugarcane bagasse
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-013-4727-y β†—  Β· Activity - Classical
16 measurements ▼
Database ID
UniProt: Q47QW5 β†—
Sequence Annotation
Explicit - Provided (first residue from UniProt sequence replace by V)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Methanol 10 % v/v 100 79 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Methanol 20 % v/v 100 69 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Ethanol 10 % v/v 100 99 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Ethanol 20 % v/v 100 85 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 79 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 38 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Acetone 10 % v/v 100 89 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Acetone 20 % v/v 100 29 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 69 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100 2 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Dimethylformamide (DMF) 10 % v/v 100 59 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Dimethylformamide (DMF) 20 % v/v 100 25 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 16 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 0 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Formaldehyde 3.7 % v/v 100 66 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent Formaldehyde 7.4 % v/v 100 1 % Direct Continuous 20 mM phosphate buffer 8 50Β°C 2 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxyl radical reaction product β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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