Carboxylesterase from Penicillium expansum GF

Enzyme Description

Species
Penicillium expansum GF β†—
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 559 amino acids
MIGFATSFLLWTAVHAVPTKSSPTVAVRNGTYNGVRSSTYNQEFFLGMPYAQQPVGNLRFTVPQSLNASWDDIRNATQYSDICVGYGTDSIWYPQSEACLTLNVIRGSSANTDSKLPVGVWIHGGGFYQGSGADERYNMSAIVENSYQIGKPFIAVTLNYRLSAWGFISSSQVSGSGNTNLGLRDQRLALQWVQENIDAFGGDPKKVTIWGESAGAMSVGYHLTAYNGGDDKLFRAGIMQSGGSIAASPGNYTTFQSKYNSSKYNSMASKVGCSDVVDSLQCLREVPFETLNTALNGTEGDSEYNFSPVVDGDLIRNWGSIQLSKHAFVKVPIMAGTNTDEGTAFGPTGINTTEQWYQYLTADGGLNFQTPPSVAKRILELYPDDPSQGIPAYLGDQRVPSNGQQWRRTSAFAGDHFMHANRRRQCEAWAETSTPAYCYRFNVHSADVPLLSGVTHFEEVAFVFHNIAGLGYHYGKPFDGVPQSYIDLSSMMTSMWASFIHDLDPNPGVVKTPVHWDSYSTNKPVDLCLDGNTTSHMEADTWRKDGIDYINSAARAFWR
T.L.R. CorrΓͺa et al. (2013) β€” Carboxyl ester hydrolase from Penicillium expansum: cloning, characterization and overproduction by Penicillium griseoroseum
Journal of Applied Microbiology  Β· doi:10.1111/jam.12215 β†—  Β· Stability - Incubation
16 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Penicillium griseoroseum PG63

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 10 % v/v 100 47 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 10 % v/v 100 54 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 10 % v/v 100 66 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 10 % v/v 100 69 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10 % v/v 100 91 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethylformamide (DMF) 10 % v/v 100 88 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100 88 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Tetrahydrofuran (THF) 10 % v/v 100 69 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 30 % v/v 100 0 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 30 % v/v 100 28 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 30 % v/v 100 52 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 30 % v/v 100 84 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 30 % v/v 100 25 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethylformamide (DMF) 30 % v/v 100 60 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30 % v/v 100 41 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Tetrahydrofuran (THF) 30 % v/v 100 53 % Digital Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 37Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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