Lipase Lip1Pc from Psychrobacter cryohalolentis K5T

Enzyme Description

Species
Psychrobacter cryohalolentis K5T β†—
Extremophile
Yes "psychrotrophic" organism
EC Number
3.1.1.3 β†—

Sequence

Length: 483 amino acids
MSNSTVLSVNTLLNKAVKTLNLMSFGQDKNPKSTDINISAEIMDIDESALQDSREDKGLSIKEKILEHHLMTNYQPHLLHYAIKSFGCLPTPILESLIKCLDGPTSKQYLHVDAHLRLILAVNSKLKTPLQLIEMSELRKRFATDAVAMQAPKVWQQASDNLLSNLKQFHKKGDSAISWQDRTIANADDGDMTIRCYQNETSDNGFGFKKEQTSNPDETVLLFFHGGGFCIGDLNTHHEFCHAICEQTGWPVISVDYRLAPEHPAPAAVRDCISAYAWLAEHCEEFGALPSRIVLAGDSAGGGLSTLMAQQIITPNKEAWLDLGDEGQKTFDILQGLPHPMAQMPLYPVTDIETDYPSWELYGEGLLLDHADVAIFDAACLENSPLPRQHILTSPMLGDNRQVCPSYVVAAELDVLRDEAFAYADQLKSYGIAVQTHTVLGAPHGFIHFMSVHQRLGQETQHIITGFANFVREIIKTRALLSA
K. A. Novototskaya-Vlasova et al. (2013) β€” Characterization of a cold-active lipase from Psychrobacter cryohalolentis K5T and its deletion mutants
Biochemistry (Moscow)  Β· doi:10.1134/S000629791304007X β†—  Β· Stability - Incubation
10 measurements ▼
Database ID
UniProt: Q1Q7W8 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm), in aqueous phase Acetonitrile 5 % v/v 100.0 8.0 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris_x0001_HCl, 100 mM NaCl, 0.5% (w/v) Triton X-100 Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm), in aqueous phase Ethanol 5 % v/v 100.0 72.0 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris_x0001_HCl, 100 mM NaCl, 0.5% (w/v) Triton X-100 Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm), in aqueous phase Methanol 5 % v/v 100.0 57.0 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris_x0001_HCl, 100 mM NaCl, 0.5% (w/v) Triton X-100 Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm), in aqueous phase Dimethyl Sulfoxide (DMSO) 5 % v/v 100.0 101.0 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris_x0001_HCl, 100 mM NaCl, 0.5% (w/v) Triton X-100 Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm), in aqueous phase Dimethylformamide (DMF) 5 % v/v 100.0 2.5 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris_x0001_HCl, 100 mM NaCl, 0.5% (w/v) Triton X-100 Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm), in aqueous phase Acetonitrile 10 % v/v 100.0 <0.5 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris_x0001_HCl, 100 mM NaCl, 0.5% (w/v) Triton X-100 Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm), in aqueous phase Ethanol 10 % v/v 100.0 31.8 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris_x0001_HCl, 100 mM NaCl, 0.5% (w/v) Triton X-100 Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm), in aqueous phase Methanol 10 % v/v 100.0 27.0 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris_x0001_HCl, 100 mM NaCl, 0.5% (w/v) Triton X-100 Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm), in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 99.0 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris_x0001_HCl, 100 mM NaCl, 0.5% (w/v) Triton X-100 Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm), in aqueous phase Dimethylformamide (DMF) 10 % v/v 100.0 <0.5 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris_x0001_HCl, 100 mM NaCl, 0.5% (w/v) Triton X-100 Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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