Alcohol dehydrogenase (gene adh2) from Halobacterium sp. NRC-1

Enzyme Description

Species
Halobacterium sp. NRC-1 β†—
Extremophile
Yes "halophilic" organism
EC Number
1.1.1.71 β†—

Sequence

Length: 347 amino acids
MRAAVYQGPGEIAVEEVPKPDIESPEDAVIRVTHTAVCGSDLWFYRGDSDREAGSRVGHEPMGIVEEVGDDVTSVAPGDRVIAPFAISCGECEFCRQGLYTSCVEDESWGSEANGGGQGEYVKCPFADGTLVRVPDRYADDEDVLESLLPLTDVMGTGHHAAVSAGVGEGDTAVVVGDGAVGLCGVLAAQRLGAERIIAMGHHEDRLELAAEFGATDTISARGDDAIERARDLTHGGANHVMECVGAASAMDSAIAIARPGGTVGYVGVPYGVEDGGLDVFTMFSDNITIRGGVAPVRAYAEELMADVLQGTLDPSPIFTKTVDLDGVPEGYAAMDDREAIKVLVKA
Ann-Kathrin Liliensiek et al. (2013) β€” Heterologous Overexpression, Purification and Characterisation of an Alcohol Dehydrogenase (ADH2) from Halobacterium sp. NRC-1
Molecular Biotechnology  Β· doi:10.1007/s12033-013-9666-4 β†—  Β· Stability - Incubation
12 measurements ▼
Database ID
UniProt: Q9HMB6 β†—
Sequence Annotation
Explicit - Provided genome NCBI Accession Number
Protein Source
Recombinant, host archaeon Haloferax volcanii (H1209-H1325)

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 5 % v/v 100 89 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Acetonitrile 5 % v/v 100 87 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Methanol 5 % v/v 100 92 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Tetrahydrofuran (THF) 5 % v/v 100 63 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100 91 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Acetonitrile 10 % v/v 100 90 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Methanol 10 % v/v 100 85 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Tetrahydrofuran (THF) 10 % v/v 100 43 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 30 % v/v 100 41 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Acetonitrile 30 % v/v 100 0 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Methanol 30 % v/v 100 6 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase Tetrahydrofuran (THF) 30 % v/v 100 0 % Direct Continuous Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl Incubation: 7.5, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM 1-Propanol Propanal 1 mM NAD(P)+ β€” Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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