Lipase from Pseudomonas sp. YY31

Enzyme Description

Species
Pseudomonas sp. YY31 β†—
Extremophile
No but "psychrotrophic" organism
EC Number
3.1.1.3 β†—

Sequence

Length: 470 amino acids
MGIFDYKNLGTEGSKALFADAMAITLYTYHNLDNGFAVGYQHNGLGLGLPATLVGALLGSTDSQGVIPGIPWNPDSEKAALDAVQKAGWTPISASTLGYAGKVDARGTFFGEKAGYTTAQVEVLGKYDDAGKLLEIGIGFRGTSGPRETLISDSIGDLVSDLLAALGPKDYAKNYAGEAFGGLLKHVAEYASAHGLSGKDVLVSGHSLGGLAVNSLADLSSSKWAGFYKDANYVAYASPTQSAGDKVLNIGYENDPVFRALDGSSFNLSSLGVHDKPHESTTDNIVSFNDHYASTLWNVMPFSIVNLPTWISHLPTGYGDGMTRILDSGFYEQMTRDSTVIVANLSDPARANTWVQDLNRNAEPHKGNTFIIGSDGNDLIQGGKGADFIEGGKGNDTIRDNSGHNTFLFSGHFGQDRIIGYQPSDKLVFKDVEGSAQYREHGGDTVISFGGDSVTLVGVSGVSGDVVFIS
Yoko Yamashiro et al. (2013) β€” A Cold-Adapted and Organic Solvent-Tolerant Lipase from a Psychrotrophic Bacterium Pseudomonas sp. Strain YY31: Identification, Cloning, and Characterization
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-013-0406-9 β†—  Β· Stability - Incubation
15 measurements ▼
Database ID
UniProt: F7J9X4 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 12.5 % v/v 100 88 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 12.5 % v/v 100 114 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 12.5 % v/v 100 153 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 12.5 % v/v 100 91 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 12.5 % v/v 100 125 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 25 % v/v 100 102 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 25 % v/v 100 105 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 25 % v/v 100 151 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 25 % v/v 100 62 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25 % v/v 100 106 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 50 % v/v 100 110 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 50 % v/v 100 93 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 50 % v/v 100 120 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 50 % v/v 100 60 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 5Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50 % v/v 100 82 % Direct Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% Gum arabic, 5 mM CaCl2, 7.5% isopropanol Incubation: 8, Assay: 8 Incubation: 5Β°C, Assay: 25Β°C 1.25 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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