Carboxypeptidase from Sulfolobus solfataricus

Enzyme Description

Species
Sulfolobus solfataricus β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.4.17.- β†—

Sequence

Length: 393 amino acids
MDLVEKLKNDVKEIEDWIIQIRRKIHENPELSYKEYSTSKLVAETLRKLGIEVEEGVGLPTAVVGKIRGNKPGKTVALRADMDALPVEETSDVEFKSKVKGVMHACGHDTHVAMLLGGAYLLVKNKDLISGEIRLIFQPAEEDGGLGGAKPMIEAGVMNGVDYVFGIHISSSYPSGVFATRKGPIMATPDAFKIVVHGKGGHGSAPHETIDPIFISLQIANAIYGITARQIDPVQPFVISITTIHSGTKDNIIPDDAEMQGTIRSLDENVRSKAKDYMRRIVSSICGIYGATCEVKFMEDVYPITVNNPEVTDEVMKILSSISTVVETEPVLGAEDFSRFLQKAPGMYFFLGTRNEKKGCIYPNHSSKFCVDEDVLKLGALAHALLAIKFSNK
Sonia Colombo et al. (1992) β€” Purification and characterization of a thermostable carboxypeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus
European Journal of Biochemistry  Β· doi:10.1111/j.1432-1033.1992.tb16934.x β†—  Β· Stability - Incubation
18 measurements ▼
Database ID
UniProt: P58156 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, archaeon Sulfolobus solfataricus

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Ethanol 99 % v/v 105 92 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Methanol 99 % v/v 96 0 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 70Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Methanol 50 % v/v 96 11 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 70Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Ethanol 99 % v/v 96 0 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 70Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Ethanol 50 % v/v 96 2 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 70Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Tetrahydrofuran (THF) 50 % v/v 96 0 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 70Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Acetonitrile 50 % v/v 96 0 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 70Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Methanol 99 % v/v 105 56 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Methanol 50 % v/v 105 113 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (0 minute at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Acetonitrile 50 % v/v 100 101 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Ethanol 50 % v/v 105 90 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Tetrahydrofuran (THF) 50 % v/v 105 19 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Acetonitrile 50 % v/v 105 93 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (0 minute at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Methanol 99 % v/v 100 105 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (0 minute at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Methanol 50 % v/v 100 122 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (0 minute at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Ethanol 99 % v/v 100 95 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (0 minute at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Ethanol 50 % v/v 100 98 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)
Stability - Incubation Activity after incubation (0 minute at 40Β°C), measured by absorbance spectrophotometry (change in N-Benzoylglycyl-L-arginine absorbance measurement, 240 nm) in aqueous phase Tetrahydrofuran (THF) 50 % v/v 100 42 % Direct Continuous Incubation: 50 mM triEthanolamine/HCl, Assay: 0.1 M potassium Mes Incubation: 7.5, Assay: 6.5 Incubation: 40Β°C, Assay: 60Β°C 0.1 mM N-Benzoylglycyl-L-arginine arginine , Benzoylglycine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase (40x dilution of organic solvent)

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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