Lipase (gene RhLip) from Rhodococcus sp. AW25M09

Enzyme Description

Species
Rhodococcus sp. AW25M09 β†—
Extremophile
Yes "psychrophlic" organism, "alkaliphilic" enzyme
EC Number
3.1.1.1 β†—

Sequence

Length: 351 amino acids
MYRSNDSNDAANAVTGTYIDPAAEWTGPGARPLVVLAPGTQGQGDQCAPSKMLNNLITYTPPLGFMVEYEVLAAYSLLSQGYGVVITDYEGLGTPGAHTYVNRASEAHAVLDAARAAQKLQGTKISADGPVAAYGYSQGGGAAAAAAELAGEYAPEMNLVGTYAGAPPADLKQTLEQVDGTILTGVIGYTLNGLLNSDPDLQPIVDGNINDAGKAMLNLVADQCVGETILNVGLHRTNEYTKTGEPLSVVLDRLPRAQEILAKNKIGERTPNAPVLIQSGTSDDIVPHGQAVGLAGDWCGKGATVQLSAAQVPAIVPGSGAGHLIPDILGLGEAQNWIKDRFYGVPAPSNC
Concetta De Santi et al. (2014) β€” A New Alkaliphilic Cold-Active Esterase from the Psychrophilic Marine Bacterium Rhodococcus sp.: Functional and Structural Studies and Biotechnological Potential
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-013-0713-1 β†—  Β· Activity - Classical
17 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (17 measurements)

17 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Diethyl Ether 20 % v/v 100 0 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Dimethylformamide (DMF) 20 % v/v 100 33 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Dimethylformamide (DMF) 15 % v/v 100 48 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Dimethylformamide (DMF) 10 % v/v 100 56 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Dimethylformamide (DMF) 5 % v/v 100 94 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 61 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15 % v/v 100 76 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 130 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100 108 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Acetonitrile 3 % v/v 100 112 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Diethyl Ether 15 % v/v 100 0 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Diethyl Ether 10 % v/v 100 130 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Diethyl Ether 5 % v/v 100 229 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Acetonitrile 20 % v/v 100 33 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Acetonitrile 15 % v/v 100 37 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Acetonitrile 10 % v/v 100 47 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in the presence of organic solvent Acetonitrile 5 % v/v 100 69 % Digital Continuous 0.1 M CAPS buffer, 3% acetonitrile 10 30Β°C 0.1 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*