Alcohol dehydrogenase from Yokenella sp. WZY002

Enzyme Description

Species
Yokenella sp. WZY002 β†—
Extremophile
No
EC Number
1.1.1.2 β†—

Sequence

Length: 339 amino acids
MSIIKSYAAKEAGSELELYEYDAGELRPEDVEVQVDYCGICHSDLSMIDNEWGFSQYPLVAGHEVIGRVAALGSAAQEKGVKVGQRVGVGWTARSCGHCDACISGNQINCLEGAVATILNRGGFAEKLRADWQWVIPLPESIDIESAGPLLCGGITVFKPLLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPSKEQEVLAMGADKVVNSRDPDALNALAGQFDLIINTVNVDLDWQPYFEALAYGGHFHTVGAVMKPLPVPAFTLIAGDRSISGSATGTPYELRKLMKFAGRSKVSPTTELFPMSQINEAIQHVRDGKARYRVVLQADF
Xiangxian Ying et al. (2014) β€” Characterization of an Allylic/Benzyl Alcohol Dehydrogenase from Yokenella sp. Strain WZY002, an Organism Potentially Useful for the Synthesis of Ξ±,Ξ²-Unsaturated Alcohols from Allylic Aldehydes and Ketones
Applied and Environmental Microbiology  Β· doi:10.1128/AEM.03980-13 β†—  Β· Activity - Classical Stability - Incubation
54 measurements ▼
Database ID
UniProt: W6CX26 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (54 measurements)

54 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Ethanol 20 % v/v 100 91 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Methanol 20 % v/v 100 49 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Ethyl Acetate 50 % v/v 100 80 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent n-Heptane 50 % v/v 100 88 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent n-Hexane 50 % v/v 100 79 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Isopropanol 40 % v/v 100 15 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Acetone 40 % v/v 100 26 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Ethanol 40 % v/v 100 16 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Methanol 40 % v/v 100 20 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100 32 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent 1-Propanol 20 % v/v 100 23 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 52 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Acetone 20 % v/v 100 126 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 60 % Digital Continuous 50 mM Tris-HCl 8 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Classical aqueous control (in %)
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Acetone 20 % v/v 100 98 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Isopropanol 20 % v/v 100 24 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (16 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Acetone 20 % v/v 100 93 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (20 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Acetone 20 % v/v 100 88 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Acetone 20 % v/v 100 88 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 20 % v/v 100 102 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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