Endoglucanase (gene mgcel44) from a mangrove soil metagenomic library

Enzyme Description

Species
Na (mangrove soil metagenomic library)
Extremophile
No
EC Number
3.2.1.4 β†—

Sequence

Length: 648 amino acids
MGLALDAAGSLYVANCGDYPCGNSNNITIFDAPATQTTATATATPTATTANVTPTPTTTTSTVTATPTATATRTPTPTATSTQVTPTTADISLSVDLSTNRHPISDAIYGIHYVEDEAFAAEIDLPVRRWGGNDTTRYNWKNSMFGNADWYFENEYKPTSADDFIAQNKRTGADSIITLPMSGWMTKDPGQPGNSATYPCSFDTRKYNYTPQALPNGLPSYDTADPKRSHCGSGVTRYENGRAVYFQGNDPTDTSFAIDSSWTSEWIAHLVQTFGAADKNGVRFYGLDNEPDLWHETHRDIFPIALTYDQIRQRAYDYAAVVKAADPAAQILGPVLMGWTYYWHSPRDGQQELWTTRPDRMSHGDVPLVPWYLQQMAEYEAQNGIRLLDYLDLHFYPQNGVDLRDAGDANLQALRLRSTRALWDPTYVDESWIADAGPDGGVVQLIPRMREWVTQNYPGTKLAITEYNWGALDHINGALTQADILGIFGREGLDLATLFDTPYGNGGNFSPQSPAAYAFRLFRNYNGAGAKFGDISVEAVSSDQEKLAVYAAERSSDNALTLLVINKSSSPLTADLSIINRPSSLSTMGQIYRYSAGAPNTIVTAADLSLAGDSTQTTFPASSITLIVVQSSPTDLPHRLYLSALNRH
Zhimao Mai et al. (2014) β€” Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library
Biotechnology Letters  Β· doi:10.1007/s10529-014-1531-4 β†—  Β· Stability - Incubation
10 measurements ▼
Database ID
UniProt: U5YC86 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 15 % v/v 100 81 % Direct Continuous Incubation: ?, Assay: Na2HPO4/citric acid buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 0.01 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 15 % v/v 100 87 % Direct Continuous Incubation: ?, Assay: Na2HPO4/citric acid buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 0.01 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetonitrile 15 % v/v 100 76 % Direct Continuous Incubation: ?, Assay: Na2HPO4/citric acid buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 0.01 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 15 % v/v 100 78 % Direct Continuous Incubation: ?, Assay: Na2HPO4/citric acid buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 0.01 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 15 % v/v 100 88 % Direct Continuous Incubation: ?, Assay: Na2HPO4/citric acid buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 0.01 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 15 % v/v 100 67 % Direct Continuous Incubation: ?, Assay: Na2HPO4/citric acid buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 0.01 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethyl Acetate 15 % v/v 100 15 % Direct Continuous Incubation: ?, Assay: Na2HPO4/citric acid buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 0.01 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 15 % v/v 100 41 % Direct Continuous Incubation: ?, Assay: Na2HPO4/citric acid buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 0.01 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 15 % v/v 100 39 % Direct Continuous Incubation: ?, Assay: Na2HPO4/citric acid buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 0.01 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Toluene 15 % v/v 100 102 % Direct Continuous Incubation: ?, Assay: Na2HPO4/citric acid buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 0.01 CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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