Ene-reductase DrER from Deinococcus radiodurans

Enzyme Description

Species
Deinococcus radiodurans β†—
Extremophile
Yes polyextremophilic organism
EC Number
1.6.99.1 β†—

Sequence

Length: 370 amino acids
MTVSSAAAPQPASPAAPLLFTPLKLRSLELPNRVVVSPMCTYSATDGVANEFHLVHLGQYALGGAGLILAEATAVSPEGRITPEDLGLWDDRQIVPLGHITDFVHQHGGHIGVQLAHAGRKASTYAPWRGKGAVPAELGGWQVIGPDENSFHDLFPTPAMMGADELRGVVDAFSAAARRAQVAGFDAVEVHAAHGYLLHQFLSPLANTRTDDYGGSFENRTRLLLEVVRAVRHVWPAHLPLFVRLSATDWAEGGWDLEQTVQLSKLLKYEGVDVLDISSGGLTAAQQIEVGPGYQVPFAAAVSRAETEISVMAVGLIETGAQAEAILQAGDADLIALGRPFLRDPHWAQRAARELGLRPVSIDQYARAGW
Frieda A. Sorgenfrei et al. (2024) β€” Solvent concentration at 50% protein unfolding may reform enzyme stability ranking and process window identification
Nature Communications  Β· doi:10.1038/s41467-024-49774-0 β†—  Β· Activity - Classical Stability - C50 Stability - Tm
164 measurements ▼
Database ID
UniProt: Q9RSD4 β†—
Sequence Annotation
Explicit - Provided UniProt Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (164 measurements)

164 measurements β€” page 6 of 9
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 40 % v/v 100.0 7.2 % Direct Continuous 50 mM sodium phosphate 7.4 35Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 45 % v/v 100.0 3.1 % Direct Continuous 50 mM sodium phosphate 7.4 35Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 5 % v/v 100.0 101.5 % Direct Continuous 50 mM sodium phosphate 7.4 40Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 10 % v/v 100.0 61.2 % Direct Continuous 50 mM sodium phosphate 7.4 40Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 15 % v/v 100.0 29.9 % Direct Continuous 50 mM sodium phosphate 7.4 40Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 20 % v/v 100.0 7.5 % Direct Continuous 50 mM sodium phosphate 7.4 40Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 25 % v/v 100.0 3.0 % Direct Continuous 50 mM sodium phosphate 7.4 40Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 4.5 % Direct Continuous 50 mM sodium phosphate 7.4 40Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 35 % v/v 100.0 4.5 % Direct Continuous 50 mM sodium phosphate 7.4 40Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 40 % v/v 100.0 7.5 % Direct Continuous 50 mM sodium phosphate 7.4 40Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 45 % v/v 100.0 3.0 % Direct Continuous 50 mM sodium phosphate 7.4 40Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 5 % v/v 100.0 80.0 % Direct Continuous 50 mM sodium phosphate 7.4 45Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 10 % v/v 100.0 34.3 % Direct Continuous 50 mM sodium phosphate 7.4 45Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 15 % v/v 100.0 2.9 % Direct Continuous 50 mM sodium phosphate 7.4 45Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 20 % v/v 100.0 8.6 % Direct Continuous 50 mM sodium phosphate 7.4 45Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 25 % v/v 100.0 8.6 % Direct Continuous 50 mM sodium phosphate 7.4 45Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 8.6 % Direct Continuous 50 mM sodium phosphate 7.4 45Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 35 % v/v 100.0 11.4 % Direct Continuous 50 mM sodium phosphate 7.4 45Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 40 % v/v 100.0 17.1 % Direct Continuous 50 mM sodium phosphate 7.4 45Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
Activity - Classical Activity measured by absorbance spectrophotometry (NAD(P)H absorbance measurement, 340 nm) in the presence of organic solvent Methanol 45 % v/v 100.0 8.6 % Direct Continuous 50 mM sodium phosphate 7.4 45Β°C 10 mM Cyclohex-2-enone Cyclohexane 0.2 mM NAD(P)H β€” Classical aqueous control (in %) | Raw activity data of reaction temperature screening
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Visualization : Activity β€” Classical

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Visualization : Stability β€” Tm

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” C50

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Structure

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