Proteinase from Thermus sp. Rt41A

Enzyme Description

Species
Thermus sp. Rt41A β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.4.21.- β†—

Sequence

Length: 278 amino acids
AVQSPATWGLDRIDQRTLPLDGRYTYTATGAGVHAYVVDTGILLSHQEFTGRIGKGYDAITPGGSAQDCNGHGTHVAGTIGGTTYGVAKGVTLHPVRVLDCNGSGSNSSVIAGLDWVTQNHVKPAVINMSLGGGASTALDTAVMNAINAGVTVVVAAGNDNRDACFYSPARVTAAITVGATTSTDYRASFSNYGRCLDLFAPGQSITSAWYTSSTATNTISGTSMATPHVTGAAALYLQWYPTATPSQVASALLYYATPNVVKNAGRYSPNLLLYTPF
Keith Peek et al. (1992) β€” Purification and characterization of a thermostable proteinase isolated from Thermus sp. strain Rt41A
European Journal of Biochemistry  Β· doi:10.1111/j.1432-1033.1992.tb17140.x β†—  Β· Stability - Incubation
12 measurements ▼
Database ID
UniProt: P80146 β†—
Sequence Annotation
Inferred - from protein name (132 first residues from UniProt sequence absent from the mature protein)
Protein Source
Purified, bacterium Thermus sp. Rt41A

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase Acetone 90 % v/v 100 124 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 4Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase Acetonitrile 90 % v/v 100 85 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 4Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase Ethanol 90 % v/v 100 111 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 4Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase 1-Butanol 90 % v/v 100 13 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 4Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase Isopropanol 90 % v/v 100 123 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 4Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase Methanol 90 % v/v 100 119 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 4Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase Acetone 90 % v/v 100 28 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 70Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase Acetonitrile 90 % v/v 100 19 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 70Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase Ethanol 90 % v/v 100 55 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 70Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase 1-Butanol 90 % v/v 100 30 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 70Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase Isopropanol 90 % v/v 100 87 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 70Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 70Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absobrance measurement, 420 nm) in aqueous phase Methanol 90 % v/v 100 4 % Direct Continuous Incubation: 50 mM Hepes/NaOH buffer, 5 mM CaCl2, Assay: 50 mM Hepes/NaOH buffer, 5 mM CaCl2 Incubation: 7.5, Assay: 7.5 Incubation: 70Β°C, Assay: 75Β°C 0.2 % (mass/volume) Azocasein Azo-peptides , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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