Ξ²-xylosidase (gene XsidB) from Geobacillus thermodenitrificans TSAS1

Enzyme Description

Species
Geobacillus thermodenitrificans TSAS1 β†—
Extremophile
Yes "extremely thermophilic" organism
EC Number
3.2.1.37 β†—

Sequence

Length: 697 amino acids
MPNNLFFNAHHSPVGAFSSFTLGFPGKSGGLDLELARPPRQNVFIGVESLHESGLYHVLPFWETSGEDESKRYDIENPDPNPQKPNILIPFAKEEIQREFHVATDTWKAGDLTFTIYSPVKAVPDPETADEEALKLALVPAVIVEMKIDNTNGTRARRAFFGFEGTDPYTSMRRIDETCPQLRGVGQGRIVGIVSKDEDVRSALHFSMEDILTAQLEENWTFGLGKVGALIVDVPAGEKKTYQFAVCFYRGGYVTAGMDTSYFYTRFFNNIEEVGLYALEKAEVLKEQSFRSNELIEKEWLSDDQKFMMAHAIRSFITAIHSCLSMKESRFGVNEGEYRMMNTFDLTVDQLFFELKMNPWTVKNVLDLYVERYSYEDGVRFPGEETEYPGGISFTHDMGVANTFLRPHHSSYELYGLSGCFSHMTHEQLVNWVLCAAVYIEQTKDWAWRDKRLAILEQCLESMVRRDHPDPEKRNGIMGLDSTRTMGGAEITTYDSLDVSLGQARNNLYLAGKCWAAYVALEKLFHDVGKEELAALAGEQAEKCAATIVSYVTDDGYIPAVMGEGNDSKIIPAIEGLVFPYFTNCHEALNKDGRFGEYIQALNAHLRYVLREGICLFPDGGWKISSTSNNSWLSKIYLCQFIARHLLGWEWDEQGKRADAAHVAWLTHPTLSIWSWSDQIIAGEIKASKYYPRGVTS
Ira Jain et al. (2014) β€” Applicability of recombinant Ξ²-xylosidase from the extremely thermophilic bacterium Geobacillus thermodenitrificans in synthesizing alkylxylosides
Bioresource Technology  Β· doi:10.1016/j.biortech.2014.07.113 β†—  Β· Activity - Classical
18 measurements ▼
Database ID
UniProt: V5N4G7 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent 1-Butanol 5 % v/v 100.0 76.78 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Benzene 10 % v/v 100.0 81.51 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Chloroform 10 % v/v 100.0 86.24 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent 1-Butanol 10 % v/v 100.0 71.56 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Ethanol 10 % v/v 100.0 70.3 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Methanol 10 % v/v 100.0 71.88 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetone 10 % v/v 100.0 48.01 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Benzene 5 % v/v 100.0 82.8 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Chloroform 5 % v/v 100.0 88.46 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetone 1 % v/v 100.0 73.18 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Ethanol 5 % v/v 100.0 72.48 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Methanol 5 % v/v 100.0 77.28 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetone 5 % v/v 100.0 72.7 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Benzene 1 % v/v 100.0 83.74 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Chloroform 1 % v/v 100.0 88.68 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent 1-Butanol 1 % v/v 100.0 80.05 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Ethanol 1 % v/v 100.0 76.67 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Methanol 1 % v/v 100.0 87.95 % Direct Continuous ? ? 70Β°C 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside D-Xylose , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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