Phenolic acid decarboxylase (gene blpad) from Bacillus licheniformis

Enzyme Description

Species

Extremophile

EC Number

4.2.1.- ↗

Sequence

Length: 166 amino acids

MNQDVKEFVGSHMIYTYENGWEYEIYIKNDHTIDYRIHSGMVGGRWVRDQKADIVKLTEGVYKVSWTEPTGTDVSLNFMPNEKRMHGIIFFPKWVHERPDITVCYQNDHIDLMEESREKYETYPKYVVPEFADITFIENAGIDNEDLISKAPYPGMTDDIRAGKRV

Hongfei Hu et al. (2014) — An organic solvent-tolerant phenolic acid decarboxylase from Bacillus licheniformis for the efficient bioconversion of hydroxycinnamic acids to vinyl phenol derivatives

Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6313-3 ↗ · Activity - Classical Stability - Incubation

45 measurements ▼

Database ID

UniProt: A0A0B4UFV2 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21

Experimental Data (45 measurements)

45 measurements — page 2 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Hexadecane	30 % v/v	100.0	86.94	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Hexadecane	20 % v/v	100.0	103.5	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Decane	50 % v/v	100.0	42.15	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Hexane	50 % v/v	100.0	99.57	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	Toluene	30 % v/v	100.0	126.22	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	Toluene	50 % v/v	100.0	128.47	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	1-Octanol	20 % v/v	100.0	106.45	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	1-Octanol	30 % v/v	100.0	98.71	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	1-Octanol	50 % v/v	100.0	90.86	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	Cyclohexane	20 % v/v	100.0	102.39	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	Cyclohexane	30 % v/v	100.0	102.77	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	Cyclohexane	50 % v/v	100.0	108.37	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Hexane	20 % v/v	100.0	93.29	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Hexane	30 % v/v	100.0	102.58	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	Toluene	20 % v/v	100.0	131.27	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Heptane	20 % v/v	100.0	99.89	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Heptane	30 % v/v	100.0	102.23	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Heptane	50 % v/v	100.0	100.7	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	Petroleum Ether	20 % v/v	100.0	103.93	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	Petroleum Ether	30 % v/v	100.0	105.56	%	Direct	Continuous	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Hexadecane

50 % v/v

100.0

127.77

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Toluene

50 % v/v

100.0

187.38

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

1-Octanol

50 % v/v

100.0

71.89

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Cyclohexane

50 % v/v

100.0

85.61

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Hexane

50 % v/v

100.0

90.97

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Heptane

50 % v/v

100.0

94.53

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Petroleum Ether

50 % v/v

100.0

96.32

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Octane

50 % v/v

100.0

105.79

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Decane

50 % v/v

100.0

124.7

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Hexadecane

50 % v/v

100.0

100.3

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Toluene

50 % v/v

100.0

186.38

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

1-Octanol

50 % v/v

100.0

70.15

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Cyclohexane

50 % v/v

100.0

214.7

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Hexane

50 % v/v

100.0

158.5

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Heptane

50 % v/v

100.0

119.64

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Petroleum Ether

50 % v/v

100.0

192.59

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Octane

50 % v/v

100.0

167.23

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Decane

50 % v/v

100.0

253.0

Direct

Continuous

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Decane

30 % v/v

100.0

92.79

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexadecane

50 % v/v

100.0

91.86

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexadecane

30 % v/v

100.0

86.94

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexadecane

20 % v/v

100.0

103.5

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Decane

50 % v/v

100.0

42.15

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexane

50 % v/v

100.0

99.57

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Toluene

30 % v/v

100.0

126.22

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Toluene

50 % v/v

100.0

128.47

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

1-Octanol

20 % v/v

100.0

106.45

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

1-Octanol

30 % v/v

100.0

98.71

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

1-Octanol

50 % v/v

100.0

90.86

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Cyclohexane

20 % v/v

100.0

102.39

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Cyclohexane

30 % v/v

100.0

102.77

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Cyclohexane

50 % v/v

100.0

108.37

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexane

20 % v/v

100.0

93.29

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexane

30 % v/v

100.0

102.58

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Toluene

20 % v/v

100.0

131.27

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Heptane

20 % v/v

100.0

99.89

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Heptane

30 % v/v

100.0

102.23

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Heptane

50 % v/v

100.0

100.7

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Petroleum Ether

20 % v/v

100.0

103.93

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Petroleum Ether

30 % v/v

100.0

105.56

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Petroleum Ether

50 % v/v

100.0

107.42

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Octane

20 % v/v

100.0

102.47

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Octane

30 % v/v

100.0

106.02

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Octane

50 % v/v

100.0

106.84

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Decane

20 % v/v

100.0

93.23

Direct

Continuous

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Phenolic acid decarboxylase (gene blpad) from Bacillus licheniformis

Enzyme Description

Sequence

Experimental Data (45 measurements)

Visualization : Activity — Classical

Visualization : Stability — Incubation

Structure

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