Xylanase from Geobacillus thermodenitrificans A333

Enzyme Description

Species
Geobacillus thermodenitrificans A333 β†—
Extremophile
Yes thermophilic organism
EC Number
3.2.1.8 β†—

Sequence

Length: 407 amino acids
MLKRSRKAIIVGFSFMLLLPLGMTNALAKTEQSYAKKPQISALHAPQLDQRYKDSFTIGAAVEPYQLLNEKDAQMLKRHFNSIVAENVMKPINIQPEEGKFNFAEADQIVRFAKKHHMDIRFHTLVWHSQVPQWFFLDKEGQPMVNETDPVKREQNKQLLLKRIETHIKTIVERYKDDIKYWDVVNEVVGDDGELRDSPWYQIAGIDYIKVAFQTARKYGGNKIKLYINDYNTEVEPKRSALYNLVKQLKEEGIPIDGIGHQSHIQIDWPSEEEIEKTIIMFADLGLDNQITELDVSMYGWPPRAYLSYDAIPEQKFLDQADRYDRLFKLYEKLSDKISNVTFWGIADNHTWLDSRADVYYDTDGNVIVDPKAPYTRVEKGNGKDAPFVFDPEYNVKPAYWAIIDHK
Loredana Marcolongo et al. (2015) β€” Properties of an alkali-thermo stable xylanase from Geobacillus thermodenitrificans A333 and applicability in xylooligosaccharides generation
World Journal of Microbiology and Biotechnology  Β· doi:10.1007/s11274-015-1818-1 β†—  Β· Stability - Incubation
41 measurements ▼
Database ID
UniProt: A4IP71 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Geobacillus thermodenitrificans A333

Experimental Data (41 measurements)

41 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Formaldehyde 15 % v/v 100 29 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Glycerol 15 % v/v 100 46 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Ethanol 15 % v/v 100 96 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Methanol 15 % v/v 100 99 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Acetone 15 % v/v 100 97 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Acetonitrile 15 % v/v 100 87 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Ethyl Acetate 15 % v/v 100 98 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Isopropanol 15 % v/v 100 92 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 15 % v/v 100 85 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Dimethylformamide (DMF) 15 % v/v 100 99 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase 1-Butanol 15 % v/v 100 114 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Formaldehyde 15 % v/v 100 42 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Glycerol 15 % v/v 100 38 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Ethanol 10 % v/v 100 107 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Ethanol 20 % v/v 100 112 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Ethanol 30 % v/v 100 100 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Ethanol 40 % v/v 100 99 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Ethanol 50 % v/v 100 98 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Ethanol 60 % v/v 100 77 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
Stability - Incubation Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric Somogyi–Nelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase Ethanol 70 % v/v 100 74 % Direct Continuous Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.005 Soluble birchwood xylan Reducing sugars β€” Yes, "under constant agitation" incubation Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one
β€Ή Prev
1 2 3
Next β€Ί

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*