Glucoside hydrolase (gene egl01) from a forest soil metagenomic library

Enzyme Description

Species
Na (forest soil metagenomic library)
Extremophile
No
EC Number
3.2.1.4 β†—

Sequence

Length: 517 amino acids
MSYMKRSISVFIACFMVAALGISGIIAPKAAAASKTPVAVNGQLTLKGTQLVNQNGKAVQLKGISSHGLQWCGDYVNKDSLKWLRDDWGINVFRAAMYTAEGGYIDNPSVKNKVKEAVEAAKELGIYVIIDWHILSDGNPNQNKAKAKEFFNEMSRLYGKTPNVIFEIANEPNGDVNWNRDIKPYAEEILSVIRKNSPKNIVIVGTGTWSQDVNDAADNQLKDGNVMYALHFYAGTHGQSLRDKADYALSKGAPIFVTEWGTSDASGNGGVYLDQSREWLKYLDSKKISWVNWNLSDKQESSAALNPGASKNGGWSQSDLSPSGKFVRDNIRSGSNGSSGDSGSNSKGSDQKDQKKDQDKPGQDSGAAANTIAVQYRAGDNNVNGNQIRPQLNIKNNSKKTVSLNRITVRYWYKTNRKGQNFDCDYAQIGCSKITHKFVQLKKAVNGADTYLEVGFKNGTLAPGADTGEIQIRLHNDGWSNYAQSGDYSFFNSNTFKNTKKITLYENGKLIWGTEPK
Mei Hua et al. (2015) β€” Direct detection, cloning and characterization of a glucoside hydrolase from forest soil
Biotechnology Letters  Β· doi:10.1007/s10529-015-1777-5 β†—  Β· Activity - Classical
10 measurements ▼
Database ID
UniProt: A0A0A7EZH7 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 1 % v/v 100 104 % Direct Continuous 200 mM Tris-HCl 7.6 50Β°C 1% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 10 % v/v 100 96 % Direct Continuous 200 mM Tris-HCl 7.6 50Β°C 1% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 1 % v/v 100 103 % Direct Continuous 200 mM Tris-HCl 7.6 50Β°C 1% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 10 % v/v 100 93 % Direct Continuous 200 mM Tris-HCl 7.6 50Β°C 1% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetonitrile 1 % v/v 100 100 % Direct Continuous 200 mM Tris-HCl 7.6 50Β°C 1% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 94 % Direct Continuous 200 mM Tris-HCl 7.6 50Β°C 1% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 1 % v/v 100 102 % Direct Continuous 200 mM Tris-HCl 7.6 50Β°C 1% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 93 % Direct Continuous 200 mM Tris-HCl 7.6 50Β°C 1% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Glycerol 1 % v/v 100 104 % Direct Continuous 200 mM Tris-HCl 7.6 50Β°C 1% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Glycerol 10 % v/v 100 100 % Direct Continuous 200 mM Tris-HCl 7.6 50Β°C 1% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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