O-phospho-l-serine sulfhydrylase from Aeropyrum pernix K1

Enzyme Description

Species
Aeropyrum pernix K1 β†—
Extremophile
Yes "hyperthermophilic" organism
EC Number
2.5.1.65 β†—

Sequence

Length: 389 amino acids
MALADISGYLDVLDSVRGFSYLENAREVLRSGEARCLGNPRSEPEYVKALYVIGASRIPVGDGCSHTLEELGVFDISVPGEMVFPSPLDFFERGKPTPLVRSRLQLPNGVRVWLKLEWYNPFSLSVKDRPAVEIISRLSRRVEKGSLVADATSSNFGVALSAVARLYGYRARVYLPGAAEEFGKLLPRLLGAQVIVDPEAPSTVHLLPRVMKDSKNEGFVHVNQFYNDANFEAHMRGTAREIFVQSRRGGLALRGVAGSLGTSGHMSAAAFYLQSVDPSIRAVLVQPAQGDSIPGIRRVETGMLWINMLDISYTLAEVTLEEAMEAVVEVARSDGLVIGPSGGAAVKALAKKAAEGDLEPGDYVVVVPDTGFKYLSLVQNALEGAGDSV
Takashi Nakamura et al. (2015) β€” Thermostability and reactivity in organic solvent of O -phospho-L-serine sulfhydrylase from hyperthermophilic archaeon Aeropyrum pernix K1
Bioscience, Biotechnology, and Biochemistry  Β· doi:10.1080/09168451.2015.1020753 β†—  Β· Activity - Classical
12 measurements ▼
Database ID
UniProt: Q9YBL2 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent Dimethylformamide (DMF) 5 % v/v 100 170 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent Dimethylformamide (DMF) 10 % v/v 100 183 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent Dimethylformamide (DMF) 20 % v/v 100 255 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent Dimethylformamide (DMF) 30 % v/v 100 297 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent Dimethylformamide (DMF) 40 % v/v 100 280 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent Dimethylformamide (DMF) 50 % v/v 100 4 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent 1,4-Dioxane 5 % v/v 100 182 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent 1,4-Dioxane 10 % v/v 100 290 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent 1,4-Dioxane 20 % v/v 100 459 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent 1,4-Dioxane 30 % v/v 100 637 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent 1,4-Dioxane 40 % v/v 100 89 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent 1,4-Dioxane 50 % v/v 100 1 % Digital Continuous 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay 7.5 80Β°C 2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide L-cysteine , Phosphate 0.2 mM PLP β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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