C-terminal truncated Ξ±-amylase from Bacillus subtilis FP-133

Enzyme Description

Species
Bacillus subtilis FP-133 β†—
Extremophile
No
EC Number
3.2.1.1 β†—

Sequence

Length: 516 amino acids
SVKNGTILHAWNWSFNTLTQNMKEIRDAGYAAIQTSPINQVKEGNQGDKSMRNWYWLYQPTSYQIGNRYLGTEQEFKDMCAAAEKYGVKVIVDAVINHTTSDYAAISDEIKRIPNWTHGNTQIKNWSDRWDVTQNSLLGLYDWNTQNTEVQTYLKRFLERALNDGADGFRYDAAKHIELPDDGNYGSRFWPNITNTSAEFQYGEILQDSASRDTAYANYMNVTASNYGHSIRSALKNRNLSVSNISHYASDVSADKLVTWVESHDTYANDEEESTWMSDDDIRLGWAVIGSRSGSTPLFFSRPEGGGNGVRFPGKSQIGDRGSALFKDQAITAVNQFHNVMAGQPEELSNPNGNNQIFMNQRGSKGVVLANAGSSSVTVNTSTKLPDGRYDNRAGAGSFQVANGKLTGTINARSAAVLYSDDIGNAPHVFLENYQTGAVHSFNDQLTVTLRANAKTAKAVYQINNGQQTAFKDGDRLTIGKGDPIGTTYNIRLTGTNGEGAERTQEYTFVKKDQAQ
Shinji Takenaka et al. (2015) β€” Characterization of the native form and the carboxy-terminally truncated halotolerant form of Ξ±-amylases from Bacillus subtilis strain FP-133
Journal of Basic Microbiology  Β· doi:10.1002/jobm.201400813 β†—  Β· Activity - Classical
11 measurements ▼
Database ID
UniProt: A0A0P0YKS0 β†—
Sequence Annotation
Explicit - Provided (45 first residues and 99 last residues from the UniProt sequence absent from the mature protein)
Protein Source
Recombinant, host bacterium Bacillus subtilis RIK1285

Experimental Data (11 measurements)

11 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25 % v/v 100.0 94.9 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Dimethylformamide (DMF) 25 % v/v 100.0 13.8 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 25 % v/v 100.0 97.0 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetonitrile 25 % v/v 100.0 87.8 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 25 % v/v 100.0 88.7 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetone 25 % v/v 100.0 115.3 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 25 % v/v 100.0 102.2 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent 1-Butanol 25 % v/v 100.0 104.3 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Chloroform 25 % v/v 100.0 0.1 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Benzene 25 % v/v 100.0 106.0 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Toluene 25 % v/v 100.0 113.0 % Direct Continuous 50 mM sodium–potassium phosphate buffer 6.8 30Β°C 5 mg/mL Starch Reducing sugars β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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