Penicillin V acylase from Pectobacterium atrosepticum

Enzyme Description

Species
Pectobacterium atrosepticum β†—
Extremophile
No
EC Number
3.5.1.11 β†—

Sequence

Length: 376 amino acids
MIKNNKRIKSTVCALSLVALTLGSAVSLACTRFVYLDPHNPDYPITARSMDWADDTETNLWIFPQELKRSGGAGQYSLEWTSKYGSVIASAFDGRKGMASTTDGVNEKGLAANVLWLAESEYPKTKPTAKKPGLSVAAWAQYVLDNFATVDEAVKSLQQEKFILVTKQVEGQKRLATLHLSLSDSSGDSAIIEYIDGKQVIHHSKNYQVMTNSPTFDQQLTLNAYWDQIGGNVMLPGTNRAADRFVRASFYVKNVNPNKLIPGVAEKGKIEKDKADLATAFSIIRNASVPYGYSLPDMPNIASTRWRTVVDHKSLQYFFESAVSPNIFWVDLKKINFAPRGGSAAKLDLGPNQSTIYSGQASGHFKPAQPFEFAGL
V.S. Avinash et al. (2015) β€” Penicillin V acylase from Pectobacterium atrosepticum exhibits high specific activity and unique kinetics
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2015.04.036 β†—  Β· Activity - Classical
18 measurements ▼
Database ID
UniProt: Q6D291 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Methanol 10 % v/v 100.0 34.7 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 27.8 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent n-Hexane 10 % v/v 100.0 77.1 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Ethyl Acetate 10 % v/v 100.0 59.7 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Acetonitrile 10 % v/v 100.0 81.4 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent 2-Butanone 10 % v/v 100.0 74.7 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Acetone 10 % v/v 100.0 103.4 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Isopropanol 10 % v/v 100.0 167.7 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Ethanol 10 % v/v 100.0 69.9 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Methanol 5 % v/v 100.0 74.9 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100.0 57.5 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent n-Hexane 5 % v/v 100.0 113.0 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Ethyl Acetate 5 % v/v 100.0 147.5 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Acetonitrile 5 % v/v 100.0 165.0 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent 2-Butanone 5 % v/v 100.0 174.0 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Acetone 5 % v/v 100.0 106.1 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Isopropanol 5 % v/v 100.0 194.6 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, p-dimethylaminobenzaldehyde (DMAB) and 6-aminopenicillanic acid reaction product absorbance measurement) in the presence of organic solvent Ethanol 5 % v/v 100.0 82.0 % Direct Continuous 100 mM acetate buffer 5 45Β°C 50 mM Penicillin V (Phenoxymethylpenicillin) 6-aminopenicillanic acid , phenoxyacetic acid β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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