Methionine adenosyltransferase from Pyrococcus furiosus

Enzyme Description

Species
Pyrococcus furiosus β†—
Extremophile
Yes "hyperthermophilic" organism
EC Number
2.5.1.6 β†—

Sequence

Length: 401 amino acids
MARNIVVEEIVRTPVEMQQVELVERKGIGHPDSIADGIAEAVSRALCREYIRRYGVILHHNTDQVEVVGGRAYPRFGGGEVVKPIYILLSGRAVELVDQELFPVHEVAIKAAKNYLKNAIRHLDVENHVIIDSRIGQGSVDLVSVFNKARENPIPLANDTSFGVGYAPLSETERLVLETEKLLNSEKFKKEYPAVGEDIKVMGLRRGNEIDLTIAAAIVDSEVATPKEYLEVKDKIKEAVEELAKEITSRKVNIYVNTADDPERGIYYITVTGTSAEAGDDGSVGRGNRVNGLITPNRHMSMEAAAGKNPVSHVGKIYNILAMLIAEDIAKTLPVEEVYVRILSQIGKPIDQPLVASIQVIPKPGHSVKEFEKDAYSIADEWLANITKVQKMILEDKISVF
Marina Porcelli et al. (2015) β€” A thermostable archaeal S -adenosylmethionine synthetase: a promising tool to improve the synthesis of adenosylmethionine analogs of biotechnological interest
Bioengineered  Β· doi:10.1080/21655979.2015.1045170 β†—  Β· Activity - Classical Stability - Incubation
52 measurements ▼
Database ID
UniProt: Q8TZW1 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (52 measurements)

52 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100 26 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Methanol 5 % v/v 100 89 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Methanol 10 % v/v 100 76 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Methanol 20 % v/v 100 65 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Methanol 30 % v/v 100 59 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Ethanol 5 % v/v 100 87 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Ethanol 10 % v/v 100 74 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Ethanol 20 % v/v 100 63 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Ethanol 30 % v/v 100 54 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Acetonitrile 10 % v/v 100 73 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Acetonitrile 20 % v/v 100 58 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Acetonitrile 30 % v/v 100 41 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100 80 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 66 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 44 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent Acetonitrile 5 % v/v 100 84 % Digital Continuous 10 mM Tris-HCl, 20 mM MgClβ‚‚ 8 50Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Classical aqueous control (in %)
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase Isopropanol 50 % v/v 100 13 % Direct Continuous Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgClβ‚‚ Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase Acetonitrile 50 % v/v 100 64 % Direct Continuous Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgClβ‚‚ Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase Dimethyl Sulfoxide (DMSO) 50 % v/v 100 100 % Direct Continuous Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgClβ‚‚ Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase
Stability - Incubation Activity after incubation (60 minutes at 60Β°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase Methanol 50 % v/v 100 16 % Direct Continuous Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgClβ‚‚ Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C ATP (Adenosine triphosphate), 5 mM L-methionine S-adenosyl-L-methionine β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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