Lipase (gene lipG1) form Acinetobacter sp. G1(2013)

Enzyme Description

Species
Acinetobacter sp. G1(2013) β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 408 amino acids
MQRRMLIAVLTSTAMLLSACHDDDHDSYHSGDNIPKNNITNPVVSTPTAYNQTDMSATAGESVVMTYKMLGINNKEVQATALVFTPKTAPPAKGWPIALWAHGTTGVADICAPSRSQLNSYIKAMIEGLLTAGYVVVAPDYEGLGEPANNELHPFLNVKSEAFSITDAVVAARNYLGNKVSNRWMTVGHSQGGHAALSAAQYQSRAQLDYKGTVAVAPASNLQLILLAGESLAAQQTDIKQKIETLASLDTFTALITAGLRNPNPSLQYSQVFKSPTDKIADNAETDCYPSLEQEFGTAMNTYAEDNADDITNYPRTQSNFMTLSPVKKFLDTDSQPLQVKISTPIIIYQGAMDTTVPKVATDTLLSGDAAKNSPITYKTNTTWDHGTAYVLNIPNILIDVQTLMPIQ
Chao Ran et al. (2015) β€” A Novel Lipase as Aquafeed Additive for Warm-Water Aquaculture
PLOS ONE  Β· doi:10.1371/journal.pone.0132049 β†—  Β· Activity - Classical
14 measurements ▼
Database ID
UniProt: A0A0D3S0I2 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 10 % v/v 100.0 109.5 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 10 % v/v 100.0 98.2 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 10 % v/v 100.0 100.3 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent 2-Octanol 10 % v/v 100.0 88.1 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent n-Heptane 10 % v/v 100.0 109.7 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Glycerol 10 % v/v 100.0 93.9 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 114.3 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 78.5 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 30 % v/v 100.0 101.7 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 30 % v/v 100.0 33.8 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent 2-Octanol 30 % v/v 100.0 41.3 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent n-Heptane 30 % v/v 100.0 110.9 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Glycerol 30 % v/v 100.0 75.5 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100.0 108.3 % Direct Continuous 50 mM Tris-HCl buffer 8 40Β°C 0.5 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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