β-galactosidase from Aspergillus oryzae

Enzyme Description

Species

Extremophile

EC Number

3.2.1.12 ↗

Sequence

Length: 1005 amino acids

MKLLSVAAVALLAAQAAGASIKHRLNGFTILEHPDPAKRDLLQDIVTWDDKSLFINGERIMLFSGEVHPFRLPVPSLWLDIFHKIRALGFNCVSFYIDWALLEGKPGDYRAEGIFALEPFFDAAKEAGIYLIARPGSYINAEVSGGGFPGWLQRVNGTLRSSDEPFLKATDNYIANAAAAVAKAQITNGGPVILYQPENEYSGGCCGVKYPDADYMQYVMDQARKADIVVPFISNDASPSGHNAPGSGTGAVDIYGHDSYPLGFDCANPSVWPEGKLPDNFRTLHLEQSPSTPYSLLEFQAGAFDPWGGPGFEKCYALVNHEFSRVFYRNDLSFGVSTFNLYMTFGGTNWGNLGHPGGYTSYDYGSPITETRNVTREKYSDIKLLANFVKASPSYLTATPRNLTTGVYTDTSDLAVTPLIGDSPGSFFVVRHTDYSSQESTSYKLKLPTSAGNLTIPQLEGTLSLNGRDSKIHVVDYNVSGTNIIYSTAEVFTWKKFDGNKVLVLYGGPKEHHELAIASKSNVTIIEGSDSGIVSTRKGSSVIIGWDVSSTRRIVQVGDLRVFLLDRNSAYNYWVPELPTEGTSPGFSTSKTTASSIIVKAGYLLRGAHLDGADLHLTADFNATTPIEVIGAPTGAKNLFVNGEKASHTVDKNGIWSSEVKYAAPEIKLPGLKDLDWKYLDTLPEIKSSYDDSAWVSADLPKTKNTHRPLDTPTSLYSSDYGFHTGYLIYRGHFVANGKESEFFIRTQGGSAFGSSVWLNETYLGSWTGADYAMDGNSTYKLSQLESGKNYVITVVIDNLGLDENWTVGEETMKNPRGILSYKLSGQDASAITWKLTGNLGGEDYQDKVRGPLNEGGLYAERQGFHQPQPPSESWESGSPLEGLSKPGIGFYTAQFDLDLPKGWDVPLYFNFGNNTQAARAQLYVNGYQYGKFTGNVGPQTSFPVPEGILNYRGTNYVALSLWALESDGAKLGSFELSYTTPVLTGYGNVESPEQPKYEQRKGAY

S. Shubhada et al. (1993) — Borate ion-assisted stabilization of beta-galactosidase from Aspergillus oryzae by polyhydroxy compounds in water-miscible organic solvents

Enzyme and Microbial Technology · doi:10.1016/0141-0229(93)90102-8 ↗ · Stability - Incubation

144 measurements ▼

Database ID

UniProt: Q2UCU3 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Commercially purchased

Experimental Data (144 measurements)

144 measurements — page 7 of 8

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10 % v/v	100	99	%	Direct	Continuous	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25 % v/v	100	101	%	Direct	Continuous	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50 % v/v	100	75	%	Direct	Continuous	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60 % v/v	100	21	%	Direct	Continuous	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10 % v/v	100	99	%	Direct	Continuous	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25 % v/v	100	100	%	Direct	Continuous	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50 % v/v	100	1	%	Direct	Continuous	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60 % v/v	100	0	%	Direct	Continuous	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10 % v/v	100	57	%	Direct	Continuous	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25 % v/v	100	2	%	Direct	Continuous	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50 % v/v	100	1	%	Direct	Continuous	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60 % v/v	100	1	%	Direct	Continuous	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10 % v/v	100	100	%	Direct	Continuous	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25 % v/v	100	100	%	Direct	Continuous	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50 % v/v	100	65	%	Direct	Continuous	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60 % v/v	100	5	%	Direct	Continuous	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10 % v/v	100	89	%	Direct	Continuous	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25 % v/v	100	69	%	Direct	Continuous	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50 % v/v	100	1	%	Direct	Continuous	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60 % v/v	100	1	%	Direct	Continuous	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

10 % v/v

100

102

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

25 % v/v

100

103

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

10 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

25 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

10 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

25 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

10 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

25 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetone

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

10 % v/v

100

101

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

25 % v/v

100

101

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

10 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

25 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

10 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

25 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

10 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

25 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Ethanol

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

10 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

25 % v/v

100

102

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

10 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

25 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

10 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

25 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

10 % v/v

100

103

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

25 % v/v

100

101

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethylformamide (DMF)

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

102

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

101

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25 % v/v

100

101

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

10 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

25 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

10 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

25 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

10 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

25 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

10 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

25 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

Acetonitrile

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

10 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

25 % v/v

100

101

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 4.6, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

10 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

25 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

50 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

60 % v/v

100

Direct

Continuous

Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

10 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

25 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

10 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

25 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

50 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

60 % v/v

100

Direct

Continuous

Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 5.7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 7, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

10 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

25 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

50 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation

Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase

1,4-Dioxane

60 % v/v

100

Direct

Continuous

Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂

Incubation: 8, Assay: 4.5

Incubation: 25°C, Assay: 25°C

2 mM o-Nitrophenyl β-D-galactoside

D-Galactose, o-Nitrophenol

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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β-galactosidase from Aspergillus oryzae

Enzyme Description

Sequence

Experimental Data (144 measurements)

Visualization : Stability — Incubation

Structure

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