Esterase (gene Est12) from a marine sediment microbial metagenomic library

Enzyme Description

Species
Na (marine sediment microbial metagenomic library)
Extremophile
No but"cold-adapted" enzyme
EC Number
3.1.1.1 β†—

Sequence

Length: 270 amino acids
MALFQCDFFSDVLGLSTSMTVILPQETTGQIGMAGGSERREHPTLFLLHGLSDDHTIWLRRTSIERYVAEMGLAVVMPAVHRSFYTDMAHGLQYWTFISEELPALARSFFPLATAREDTFVAGLSMGGYGALKLGMRHPERFAAAASLSGALDITFDPAEHIAMEDDVWVAEQRNIFGDLAALPGSDHDLFALAERMAQSDGPVPKLYQCCGTEDFLYEDNVRFRDHVRGLGLDFMYEESPGEHEWGYWDAQIQRVLAWLPLRPPGTAPA
Yongfei Hu et al. (2015) β€” Structural and functional analysis of a low-temperature-active alkaline esterase from South China Sea marine sediment microbial metagenomic library
Journal of Industrial Microbiology and Biotechnology  Β· doi:10.1007/s10295-015-1653-2 β†—  Β· Activity - Classical
15 measurements ▼
Database ID
UniProt: B8Y562 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 1 % v/v 100 100 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100 98 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 74 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 1 % v/v 100 101 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 5 % v/v 100 72 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 10 % v/v 100 43 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 10 % v/v 100 80 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 20 % v/v 100 64 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 40 % v/v 100 14 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 10 % v/v 100 53 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 20 % v/v 100 27 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 40 % v/v 100 0 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 10 % v/v 100 61 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 20 % v/v 100 18 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 40 % v/v 100 0 % Direct Continuous 50 mM Tris-HCl buffer, 0.1% arabic gum 8 40Β°C 0.01 mM p-Nitrophenyl hexanoate caproid acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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