Lipase (gene lipW) from Bacillus subtilis

Enzyme Description

Species
Bacillus subtilis β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 210 amino acids
MKKVLMAFIICLSLILSVLAAPPSGAKAESVHNPVVLVHGISGASYNFFAIKNYLISQGWQSNKLYAIDFYDKTGNNLNNGPQLASYVDRVLKETGAKKVDIVAHSMGGANTLYYIKYLGGGNKIQNVVTLGGANGLVSSTALPGTDPNQKILYTSIYSLNDQIVINSLSRLQGARNIQLYGIGHIGLLSNSQVNGYIKEGLNGGGLNTN
Jyoti Khurana et al. (2015) β€” New Insight into Old Bacillus Lipase: Solvent Stable Mesophilic Lipase Demonstrating Enzyme Activity towards Cold
Microbial Physiology  Β· doi:10.1159/000439276 β†—  Β· Stability - Incubation
28 measurements ▼
Database ID
UniProt: B8YLY0 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli JM109

Experimental Data (28 measurements)

28 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Hexane 50 % v/v 100 225 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Isopropanol 80 % v/v 100 58 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Isopropanol 50 % v/v 100 98 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 80 % v/v 100 18 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50 % v/v 100 50 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Tetrahydrofuran (THF) 80 % v/v 100 89 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Tetrahydrofuran (THF) 50 % v/v 100 98 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Heptane 80 % v/v 100 87 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Heptane 50 % v/v 100 96 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Toluene 80 % v/v 100 44 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Toluene 50 % v/v 100 60 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Benzene 80 % v/v 100 154 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Benzene 50 % v/v 100 190 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Hexane 80 % v/v 100 187 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (2 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Hexane 50 % v/v 100 160 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (2 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Isopropanol 80 % v/v 100 66 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (2 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Isopropanol 50 % v/v 100 100 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (2 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 80 % v/v 100 31 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (2 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50 % v/v 100 80 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (2 hours at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Tetrahydrofuran (THF) 80 % v/v 100 104 % Direct Continuous Incubation: ?, Assay: 40 mM phosphate buffer, 2 mM sodium deoxycholate Incubation: ?, Assay: 8 Incubation: ?, Assay: 37Β°C 0.2 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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