Cytochrome c from Equus caballus (horse)

Enzyme Description

Species

Equus caballus (horse) ↗

Extremophile

EC Number

1.11.1.5 ↗

Sequence

Length: 105 amino acids

MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFTYTDANKNKGITWKEETLMEYLENPKKYIPGTKMIFAGIKKKTEREDLIAYLKKATNE

Rafael Vazquez-Duhalt et al. (1993) — Effect of water-miscible organic solvents on the catalytic activity of cytochrome c

Enzyme and Microbial Technology · doi:10.1016/0141-0229(93)90169-3 ↗ · Activity - Classical Activity - Michaelis-Menten

72 measurements ▼

Database ID

UniProt: P00004 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Commercially purchased

Experimental Data (72 measurements)

72 measurements — page 2 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	90 % v/v	0.87857	0.59285714	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	80 % v/v	0.87857	0.2857142	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	70 % v/v	0.87857	0.1999	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	65 % v/v	0.87857	0.721428	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	60 % v/v	0.87857	0.75	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	50 % v/v	0.87857	0.73571	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	40 % v/v	0.87857	0.72142	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	30 % v/v	0.9615	2.019	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20 % v/v	0.87857	0.72142	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	100 % v/v	1.38541	0.0	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	80 % v/v	1.38541	0.0208333	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	60 % v/v	1.38541	0.114	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	55 % v/v	1.38541	0.18749999	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	50 % v/v	1.38541	0.854166	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	45 % v/v	1.38541	0.9166	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	40 % v/v	1.38541	1.28125	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	20 % v/v	0.9615	1.846	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	20 % v/v	1.768	1.66745	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	30 % v/v	1.768	2.3973328	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	40 % v/v	1.768	2.607	deltaA603/(min*nmol)	Digital	Continuous	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

1.72972

2.36036

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% acetonitrile | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Dimethylformamide (DMF)

30 % v/v

1.38541

1.40625

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Dimethylformamide (DMF)

20 % v/v

1.38541

1.385416

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Acetonitrile

80 % v/v

1.72972

0.036

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Acetonitrile

70 % v/v

1.72972

0.25225

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Acetonitrile

60 % v/v

1.72972

1.15315

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Acetonitrile

50 % v/v

1.72972

1.333333

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Acetonitrile

40 % v/v

1.72972

1.765765

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

1.72972

2.0

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Dimethylformamide (DMF)

35 % v/v

1.38541

1.3541

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Isopropanol

100 % v/v

0.9615

0.0769

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Isopropanol

80 % v/v

0.9615

0.2499999

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Isopropanol

75 % v/v

0.9615

0.326923

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Isopropanol

70 % v/v

0.9615

0.96153

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Isopropanol

65 % v/v

0.9615

2.61538461

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Isopropanol

60 % v/v

0.9615

2.34615384

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Isopropanol

50 % v/v

0.9615

2.365384615

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Isopropanol

40 % v/v

0.9615

2.4615384

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

30 % v/v

0.87857

0.84285

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100 % v/v

0.87857

0.3428571428

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

90 % v/v

0.87857

0.59285714

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

80 % v/v

0.87857

0.2857142

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

70 % v/v

0.87857

0.1999

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

65 % v/v

0.87857

0.721428

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

60 % v/v

0.87857

0.75

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

50 % v/v

0.87857

0.73571

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

40 % v/v

0.87857

0.72142

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Isopropanol

30 % v/v

0.9615

2.019

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

0.87857

0.72142

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100 % v/v

1.38541

0.0

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Dimethylformamide (DMF)

80 % v/v

1.38541

0.0208333

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Dimethylformamide (DMF)

60 % v/v

1.38541

0.114

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Dimethylformamide (DMF)

55 % v/v

1.38541

0.18749999

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Dimethylformamide (DMF)

50 % v/v

1.38541

0.854166

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Dimethylformamide (DMF)

45 % v/v

1.38541

0.9166

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Dimethylformamide (DMF)

40 % v/v

1.38541

1.28125

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Isopropanol

20 % v/v

0.9615

1.846

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

20 % v/v

1.768

1.66745

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

30 % v/v

1.768

2.3973328

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

40 % v/v

1.768

2.607

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

50 % v/v

1.768

3.259

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

60 % v/v

1.768

2.76874

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

70 % v/v

1.768

2.563

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

80 % v/v

1.768

0.87756

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

85 % v/v

1.768

0.33339

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

90 % v/v

1.768

0.2829

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

100 % v/v

1.768

0.051

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Classical

Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent

Methanol

75 % v/v

1.768

1.421839

deltaA603/(min*nmol)

Digital

Continuous

6 mM phosphate buffer

6.1

Room temperature

? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Activity - Michaelis-Menten

Km (mM)

Tetrahydrofuran (THF)

50 % v/v

21.5

1.2

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

Km (mM)

Tetrahydrofuran (THF)

40 % v/v

21.5

1.7

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

Km (mM)

Tetrahydrofuran (THF)

30 % v/v

21.5

6.2

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

Km (mM)

Tetrahydrofuran (THF)

20 % v/v

21.5

23.7

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat (deltaA603/(min*nmol))

Tetrahydrofuran (THF)

90 % v/v

15.5

3.7

deltaA603/(min*nmol)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat (deltaA603/(min*nmol))

Tetrahydrofuran (THF)

80 % v/v

15.5

2.6

deltaA603/(min*nmol)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat (deltaA603/(min*nmol))

Tetrahydrofuran (THF)

70 % v/v

15.5

0.3

deltaA603/(min*nmol)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat (deltaA603/(min*nmol))

Tetrahydrofuran (THF)

60 % v/v

15.5

1.9

deltaA603/(min*nmol)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat (deltaA603/(min*nmol))

Tetrahydrofuran (THF)

50 % v/v

15.5

2.1

deltaA603/(min*nmol)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat (deltaA603/(min*nmol))

Tetrahydrofuran (THF)

40 % v/v

15.5

2.7

deltaA603/(min*nmol)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat (deltaA603/(min*nmol))

Tetrahydrofuran (THF)

20 % v/v

15.5

21.0

deltaA603/(min*nmol)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat (deltaA603/(min*nmol))

Tetrahydrofuran (THF)

30 % v/v

15.5

6.5

deltaA603/(min*nmol)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

Km (mM)

Tetrahydrofuran (THF)

60 % v/v

21.5

1.3

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

Km (mM)

Tetrahydrofuran (THF)

70 % v/v

21.5

0.7

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

Km (mM)

Tetrahydrofuran (THF)

80 % v/v

21.5

11.8

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

Km (mM)

Tetrahydrofuran (THF)

90 % v/v

21.5

10.8

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat/Km

Tetrahydrofuran (THF)

20 % v/v

0.72

0.88

deltaA603/(min*nmol*mM)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat/Km

Tetrahydrofuran (THF)

30 % v/v

0.72

1.05

deltaA603/(min*nmol*mM)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat/Km

Tetrahydrofuran (THF)

40 % v/v

0.72

1.6

deltaA603/(min*nmol*mM)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat/Km

Tetrahydrofuran (THF)

50 % v/v

0.72

1.71

deltaA603/(min*nmol*mM)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat/Km

Tetrahydrofuran (THF)

60 % v/v

0.72

1.46

deltaA603/(min*nmol*mM)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat/Km

Tetrahydrofuran (THF)

70 % v/v

0.72

0.39

deltaA603/(min*nmol*mM)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat/Km

Tetrahydrofuran (THF)

80 % v/v

0.72

0.22

deltaA603/(min*nmol*mM)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Michaelis-Menten

kcat/Km

Tetrahydrofuran (THF)

90 % v/v

0.72

0.34

deltaA603/(min*nmol*mM)

Direct

Continuous

6 mM phosphate buffer

6.1

Room temperature

H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride

H2O, Oxidized pinacyanol

—

Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity — Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Cytochrome c from Equus caballus (horse)

Enzyme Description

Sequence

Experimental Data (72 measurements)

Visualization : Activity — Classical

Visualization : Activity — Michaelis-Menten

Structure

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