Amylopullulanase (gene PulSS4) from a gut microflora of Hermetia illucens metagenomic library

Enzyme Description

Species
Na (gut microflora of Hermetia illucens metagenomic library)
Extremophile
No
EC Number
3.2.1.41 β†—

Sequence

Length: 1484 amino acids
MLLAVTEIVFCYQGGSMRKRSLYAMILTALTLLVGIVPLSVVNAEEANILTIYYIDDNYEANELSVWYWGKNADPINQNEYSTDSKKFTVGEKYSQVDLNLKNYEAFELGMILLHNSTKFQEEDIKIDIPAGTTEVWINSELEYFFEDPNQEVNQDFIRIHFKTDSINLEDVKLWGWNDFADGYKEGNWPADHRLATGVDNGFVYFDMLLKEDAQTIGFLFVNSDLGFQTNDYIYTIDSMNRNVYVQEGDPSVYHSSEMTNAELGSAVVLSENQIRLNLGNNHGYDEASLKNTFKLYADETEIMYEIDELSQRVGIISVDNPIDIKQDWEIKYDNRSVSLYNEWNYIDSVYATEKDLGLTWENQKATLNFWSPSATSVEVVIYSKDDQSQEIARIPMSEIDNVWTKTLEPSDVYDTTLQNYYYQYYITRDNKGLYVLDPYAKSMATWNGLDKAGCDNTQPQCIGKGAFVNVSDIGVSLDYADIEGFESSEDAIIYEVHVRDFTSDETIEDELINQFGTFSAFIEKLDYIEDLGVTHIQLLPVMSYYNINENNSSERTTDYSLQGESSNYNWGYDPHSYFSLTGEYSLNSDEASLRIEEYKKMIDEIHSRGMGVIMDVVYNHTAQVSIFEDLEPNYYHFMDSAGISKTSFGGGRLGTTHAMASRVLVDSITYWTDEFKIDGFRFDMMGDHDAASIQLAYDKASTLNPNTVFLGEGWTTYAGDDDYQGVQPADQSWMKLTDDVGVFSDEIRNELKSGFGSEGQPRFLTGGARNISTIFNNIKAQPGNFIADSPQDVITYIEAHDNLTLHDVIAQSIKKDPSTDESEILQRQRLGNLMVLTSQGTAFLHAGQEYGRTKQVIDDNYKTVQTLCPGSGDTYLHKGTCMTDDQGTPFEYPYFIHDSYNSSDIVNRFDWSKVRDETEYSENVKTKEYTRGLIELRRSTDAFTLSSKSDVDSKVKQLSLSNQSSEDLILGYYIDDIDYRYYVLLNADDTTRTLGVDYTTLEILKSSDVLVDSNQAGINPIATPVGISFNSDVSIDALSGVVLRISQDDLETIGYTKNETSKIDDGTVLHTWNWSFKTISDNLEDIKEAGYTAIQTSPVQPTKEIASDGSKWWLLYQPIDFSLGNQLGTEEEFVEMVTKAKELDIEIIVDVVLNHMANEGGDELSCVPNSNIRAELLNSNYWHQNGCSIDYSNRTQVTQNGIGLPDLNTSNHELQDIMIDYLNYLEDLGVDGFRFDAAKHIELPNDSGVTSDFWPNVLGSLQDTSYVYGEVIQDTVDELTKYAAFMDVTATNYGFTLRNAVTNKNVNGLVNYQANNLGAKDLVTWVESHDNYANDDEQSTYLSDEDIILAWSLLASRKENTPLFFARPDGSKFTDEIGKSGSELWKDPTIKAINDFHNKFKSEDEYILQPNDTTLIITRGVKGMVIVNVGDTSVNLNQNVFMGDGVYENTLDTTQTYTIENSKLVGTVEARSVIIIEFDETKW
Young-Seok Lee et al. (2016) β€” Identification of a novel alkaline amylopullulanase from a gut metagenome of Hermetia illucens
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2015.10.067 β†—  Β· Stability - Incubation
7 measurements ▼
Database ID
UniParc: UPI00032B72F7 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (7 measurements)

7 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100 94 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 9, Assay: 9 Incubation: 35Β°C, Assay: 40Β°C 0.5% (w/v) Pullulan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethylformamide (DMF) 10 % v/v 100 88 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 9, Assay: 9 Incubation: 35Β°C, Assay: 40Β°C 0.5% (w/v) Pullulan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 10 % v/v 100 82 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 9, Assay: 9 Incubation: 35Β°C, Assay: 40Β°C 0.5% (w/v) Pullulan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetonitrile 10 % v/v 100 82 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 9, Assay: 9 Incubation: 35Β°C, Assay: 40Β°C 0.5% (w/v) Pullulan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 10 % v/v 100 79 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 9, Assay: 9 Incubation: 35Β°C, Assay: 40Β°C 0.5% (w/v) Pullulan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 10 % v/v 100 75 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 9, Assay: 9 Incubation: 35Β°C, Assay: 40Β°C 0.5% (w/v) Pullulan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 10 % v/v 100 52 % Digital Continuous Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 9, Assay: 9 Incubation: 35Β°C, Assay: 40Β°C 0.5% (w/v) Pullulan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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