Esterase (gene estW) from Streptomyces lividans TK64

Enzyme Description

Species
Streptomyces lividans TK64 β†—
Extremophile
No but "thermostable" enzyme
EC Number
3.1.1.1 β†—

Sequence

Length: 289 amino acids
MSFLNPRIPGVNSDDVVTDITEAPHRLGAVVQAGGAQVIATVDVQAGEDDWNTPPSEEHPRPVVLVHGTFGNRGYTWNTAVPLLRRHGHRVFRLDYGQHGNPLIFGLGDIKHSARQLADFVDEVLRRTGAQQVDLVGFSQGGMMPRYYLNALGGGPKVHNFVGISPSNHGVTAQGLMNLARQIPGAVELLEQGAVGEVVPAWPQLQHDHLFQRELADLGETTEGVRHTVIATQYDDVVTPYTSCALAKTEGCYVNNIVLQDIDPDDHTPHVSMPYNATVLNEVLKALAD
Baojuan Wang et al. (2016) β€” Characterization of a novel highly thermostable esterase from the Gram-positive soil bacterium Streptomyces lividans TK64
Biotechnology and Applied Biochemistry  Β· doi:10.1002/bab.1465 β†—  Β· Activity - Classical
14 measurements ▼
Database ID
UniProt: F2Q6J2 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli Rosetta (DE3)

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 15 % v/v 100.0 66.2 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 15 % v/v 100.0 74.9 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 15 % v/v 100.0 64.7 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isopropanol 15 % v/v 100.0 92.0 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 15 % v/v 100.0 51.1 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15 % v/v 100.0 70.8 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 15 % v/v 100.0 60.6 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 33.2 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 30 % v/v 100.0 35.5 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100.0 12.8 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isopropanol 30 % v/v 100.0 60.4 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 30 % v/v 100.0 19.3 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100.0 41.3 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 30 % v/v 100.0 24.4 % Direct Continuous 50 mM Tris-HCl 8 37Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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