Protease from Stenotrophomonas maltophilia FF11

Enzyme Description

Species
Stenotrophomonas maltophilia FF11 β†—
Extremophile
No but "psychrotolerant", "halo-tolerant" enzyme
EC Number
3.4.- β†—

Sequence

Length: 617 amino acids
MIKKQNLRINVLAAAVLSMTAVGAVHAAGLPTREPVRQASTAQPGAERIIVKYRAGAAAATDRSAKLSTVQSALTRASLSGGTSRASTLGPQVVRKLATGADLIRVQGRLAPAELQRVLKELQADPSVQYAEADVKLRRTELRAGDVQPALVPNDPFYQQNQWHLHNAVGGINAPAAWDVSQGEGIVVAVIDTGILPQHPDLVGNLLEGYDFISDAETSRRPTNDRVPGAQDYGDWVENDNECYDGSLAEDSSWHGTHVAGTVAEQTNNGVGMAGVAYKAKVLPVRVLGKCGGYLSDIADAVVWASGGTVTGIPANTNPAEIINMSLGGSGACGSTYQDAINGAISRGTTVVVAAGNEADNASKYRPASCEGVVTVGATRITGGITYYSNYGTRVDLSGPGGGGSVDGNPGGYIWQTGSNAATTPESGTPGYMGMGGTSMASPHVAAVAALVQSALIAKGKDPLTPAAMRTLLKETARPFPVAIPAATPIGTGILDAKAALAKALEEPCTENCGPVATPLTNKAAVGGLSGAAGSSKLYSFEAAAGKQFSVITYGGTGNVSVYVAEGREPSASDNDAKSTRPGTSETVRVTKPVAATYYIKVVGEAAYNGVSILATQ
Qingling Wang et al. (2016) β€” Characterization of a salt-activated protease with temperature-dependent secretion in Stenotrophomonas maltophilia FF11 isolated from frozen Antarctic krill
Journal of Industrial Microbiology and Biotechnology  Β· doi:10.1007/s10295-016-1749-3 β†—  Β· Activity - Classical
18 measurements ▼
Database ID
UniProt: A0A120H335 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Purified, bacterium Stenotrophomonas maltophilia FF11

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Ethanol 50 % v/v 100.0 89.1 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Isooctane 50 % v/v 100.0 100.35 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Isooctane 30 % v/v 100.0 108.3 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent n-Hexane 50 % v/v 100.0 100.5 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent n-Hexane 30 % v/v 100.0 147.4 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Isopropanol 50 % v/v 100.0 73.1 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Isopropanol 30 % v/v 100.0 88.2 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Acetone 50 % v/v 100.0 134.1 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Acetone 30 % v/v 100.0 110.0 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100.0 179.2 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Ethanol 30 % v/v 100.0 104.2 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent 1,4-Dioxane 50 % v/v 100.0 148.4 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent 1,4-Dioxane 30 % v/v 100.0 138.6 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Methanol 50 % v/v 100.0 99.7 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 115.7 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Dimethylformamide (DMF) 50 % v/v 100.0 153.9 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Dimethylformamide (DMF) 30 % v/v 100.0 189.2 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 50 % v/v 100.0 135.4 % Direct Continuous 20 mM Tris-HCl 8.3 55Β°C 0.01 Azocasein Azopeptides , Peptides β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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