Protease from Bacillus amyloliquefaciens CMW1

Enzyme Description

Species
Bacillus amyloliquefaciens CMW1 β†—
Extremophile
No
EC Number
3.4.21.62 β†—

Sequence

Length: 382 amino acids
MRGKKVWISLLFALALIFTMAFGSTSSAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVISEKGGKVQKQFKYVDAASATLNEKAVKELKKDPSVAYVEEDHVAHAYAQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETNPFQDNNSHGTHVAGTVAALNNSIGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGTSGSSSTVGYPGKYPSVIAVGAVDSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENTTTKLGDSFYYGKGLINVQAAAQ
Atsushi Kurata et al. (2016) β€” Properties of an ionic liquid-tolerant Bacillus amyloliquefaciens CMW1 and its extracellular protease
Extremophiles  Β· doi:10.1007/s00792-016-0832-z β†—  Β· Activity - Classical
26 measurements ▼
Database ID
UniProt: D4PBI3 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Purified, bacterium Bacillus amyloliquefaciens CMW1

Experimental Data (26 measurements)

26 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Isopropanol 50 % v/v 90 14 % Digital Continuous 100 mM glycine-NaOH buffer, without CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent 1-Octanol 50 % v/v 90 66 % Digital Continuous 100 mM glycine-NaOH buffer, without CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent 1-Octanol 50 % v/v 100 72 % Digital Continuous 100 mM glycine-NaOH buffer, with 2 mM CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Toluene 50 % v/v 90 52 % Digital Continuous 100 mM glycine-NaOH buffer, without CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Toluene 50 % v/v 100 72 % Digital Continuous 100 mM glycine-NaOH buffer, with 2 mM CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Chloroform 50 % v/v 90 36 % Digital Continuous 100 mM glycine-NaOH buffer, without CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Chloroform 50 % v/v 100 44 % Digital Continuous 100 mM glycine-NaOH buffer, with 2 mM CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Butyl Acetate 50 % v/v 90 79 % Digital Continuous 100 mM glycine-NaOH buffer, without CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Butyl Acetate 50 % v/v 100 107 % Digital Continuous 100 mM glycine-NaOH buffer, with 2 mM CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Diethyl Ether 50 % v/v 90 106 % Digital Continuous 100 mM glycine-NaOH buffer, without CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Diethyl Ether 50 % v/v 100 111 % Digital Continuous 100 mM glycine-NaOH buffer, with 2 mM CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Ethyl Acetate 50 % v/v 90 40 % Digital Continuous 100 mM glycine-NaOH buffer, without CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Ethyl Acetate 50 % v/v 100 54 % Digital Continuous 100 mM glycine-NaOH buffer, with 2 mM CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Formamide 50 % v/v 100 35 % Digital Continuous 100 mM glycine-NaOH buffer, with 2 mM CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Isopropanol 50 % v/v 100 23 % Digital Continuous 100 mM glycine-NaOH buffer, with 2 mM CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Ethanol 50 % v/v 90 9 % Digital Continuous 100 mM glycine-NaOH buffer, without CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Ethanol 50 % v/v 100 15 % Digital Continuous 100 mM glycine-NaOH buffer, with 2 mM CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Acetonitrile 50 % v/v 90 8 % Digital Continuous 100 mM glycine-NaOH buffer, without CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Acetonitrile 50 % v/v 100 21 % Digital Continuous 100 mM glycine-NaOH buffer, with 2 mM CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent Methanol 50 % v/v 90 25 % Digital Continuous 100 mM glycine-NaOH buffer, without CaCl2 9 55Β°C 0.25% (w/v) Azocasein Azopeptides , Peptides β€” Yes, "vortex used" Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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