X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis

Enzyme Description

Species
Lactococcus lactis β†—
Extremophile
No
EC Number
3.4.14.5 β†—

Sequence

Length: 763 amino acids
MRFNHFSIVDKNFDEQLAELDQLGFRWSVFWDEKKILKDFLIQSPSDMTALQATAELDVIEFLKSSIELDWEIFWNIALQLLDFVPNFDFEIGKAFEYAKNSNLPQIEAEMTTENIISAFYYLLCTRRKTGMILVEHWVSEGLLPLDNHYHFFNDKSLATFDSSLLEREVLWVESPVDSEQRGENDLIKIQIIRPKSTEKLPVVMTASPYHLGINDKANDLALHDMNVELEEKTSHEIHVEQKLPQKLSAKAKELPIVDKAPYRFTHGWTYSLNDYFLTRGFASIYVAGVGTRSSDGFQTSGDYQQIYSMTAVIDWLNGRARAYTSRKKTHEIKASWANGKVAMTGKSYLGTMAYGAATTGVEGLELILAEAGISSWYNYYRENGLVRSPGGFPGEDLDVLAALTYSRNLDGADFLKGNAEYEKRLAEMTAALDRKSGDYNQFWHDRNYLINTDKVKADVLIVHGLQDWNVTPEQAYNFWKALPEGHAKHAFLHRGAHIYMNSWQSIDFSETINAYFVAKLLDRDLNLNLPPVILQENSKDQVWTMMNDFGANTQIKLPLGKTAVSFAQFDNNYDDETFKKYSKDFNVFKKDLFENKANEAVIDLELPSMLTINGPVELELRLKLNDTKGFLSAQILDFGQKKRLEDKVRVKDFKVLDRGRNFMLDDLVELPLVESPYQLVTKGFTNLQNQSLLTVSDLKADEWFTIKFELQPTIYHLEKADKLRVILYSTDFEHTVRDNRKVTYEIDLSQSKLIIPIESVKN
J.F. Chich et al. (1995) β€” Preparation of Bacterial X-Prolyl Dipeptidyl Aminopeptidase and Its Stabilization by Organic Cosolvents
Analytical Biochemistry  Β· doi:10.1006/abio.1995.1036 β†—  Β· Stability - Incubation
80 measurements ▼
Database ID
UniProt: P22346 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Lactococcus lactis

Experimental Data (80 measurements)

80 measurements β€” page 2 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Ethanol 75 % v/v 100 10 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase 1,4-Dioxane 90 % v/v 100 6 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase 1,4-Dioxane 75 % v/v 100 8 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase 1,4-Dioxane 50 % v/v 100 8 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase 1,4-Dioxane 25 % v/v 100 10 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Acetonitrile 90 % v/v 100 0 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Acetonitrile 75 % v/v 100 0 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Acetonitrile 50 % v/v 100 3 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Acetonitrile 25 % v/v 100 50 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Ethanol 90 % v/v 100 10 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Methanol 25 % v/v 100 52 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Ethanol 50 % v/v 100 12 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Ethanol 25 % v/v 100 24 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 30Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 4Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase 1,4-Dioxane 90 % v/v 100 9 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 4Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 4Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase 1,4-Dioxane 75 % v/v 100 12 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 4Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 4Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase 1,4-Dioxane 50 % v/v 100 81 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 4Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 4Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase 1,4-Dioxane 25 % v/v 100 110 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 4Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 4Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Acetonitrile 90 % v/v 100 7 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 4Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 4Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Acetonitrile 75 % v/v 100 26 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 4Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation Activity after incubation (1 hour at 4Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase Acetonitrile 50 % v/v 100 44 % Digital Continuous Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl 8 Incubation: 4Β°C, Assay: 37Β°C 0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride L-alanyl-L-proline , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
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Visualization : Stability β€” Incubation

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Structure

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