Esterase (gene Pf_Est) from Pyrococcus furiosus COM1

Enzyme Description

Species
Pyrococcus furiosus COM1 β†—
Extremophile
Yes "hyperthermophilic" organism
EC Number
3.1.1.1 β†—

Sequence

Length: 247 amino acids
ASITFEMFIPQLNTTRRIWIYLPPDYNYSTKRYPVLYMFDGQNLFDAATSFAGEWGVDEALEKLYKEKNFSIIVVGIDNGGDRRIDEYAPWVNRDYRRGGLGNATVKFIVETLKPYIDAHYRTDPEKTGIMGSSLGGLMAIYAGFSYPEVFRYVGAMSSAFWFNPEIYDFVREAKKGPEKIYIDWGTNEGRNPKAFSESNEKMVKILKEKGYREEFNLKVVIDKGGLHNEYYWGKRFPQAVLWLFEE
F. Mandelli et al. (2016) β€” Characterization and Low-Resolution Structure of an Extremely Thermostable Esterase of Potential Biotechnological Interest from Pyrococcus furiosus
Molecular Biotechnology  Β· doi:10.1007/s12033-016-9975-5 β†—  Β· Stability - Incubation
12 measurements ▼
Database ID
UniProt: I6UZ00 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number (159 first residues from UniProt sequence absent from the mature protein, and residues AS added at N-terminal end)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 5 % v/v 100.0 90.7 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 50 % v/v 100.0 52.7 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isobutanol 5 % v/v 100.0 118.3 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isobutanol 50 % v/v 100.0 63.9 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 5 % v/v 100.0 115.2 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 50 % v/v 100.0 39.5 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 5 % v/v 100.0 102.8 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 50 % v/v 100.0 51.5 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Hexanol 5 % v/v 100.0 99.8 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Hexanol 50 % v/v 100.0 111.2 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dichloromethane 5 % v/v 100.0 213.0 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dichloromethane 50 % v/v 100.0 369.0 % Direct Continuous Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v Incubation: 8, Assay: 8 Incubation: 60Β°C, Assay: 80Β°C 2 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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