Esterase from Shewanella sp.

Enzyme Description

Species

Shewanella sp. ↗

Extremophile

No but "cold-adapted", "halotolerant" enzyme

EC Number

3.1.1.1 ↗

Sequence

Length: 223 amino acids

MSSATLDAVVVEPREPATACVIWLHGLGDSGDGFAPVVPALGLPADHSIRFVFPHAPEQAVTVNGGYVMRAWYDIKSLNLHDRADMPGVLQSERLVRQLIEQQVAQGIAANRIVLAGFSQGGVMSLFTALRYPEALAGIMALSCYLPGGEQLPEDVSSANRDTPILQCHGEQDDVVALSAGLMAKEALEQGGYRLEWHTFPMAHGVVPQELNLISQWLQARLS

Yian Hang et al. (2016) — Mutational analysis and stability characterization of a novel esterase of lipolytic enzyme family VI from Shewanella sp

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.09.032 ↗ · Activity - Classical

24 measurements ▼

Database ID

UniProt: A0A380BYZ7 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	20 % v/v	100.0	1.88	%	Direct	Continuous	50 mM phosphate-citrate buffer	8	30°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	10 % v/v	100.0	11.04	%	Direct	Continuous	50 mM phosphate-citrate buffer	8	30°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	30 % v/v	100.0	2.55	%	Direct	Continuous	50 mM phosphate-citrate buffer	8	30°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	20 % v/v	100.0	18.24	%	Direct	Continuous	50 mM phosphate-citrate buffer	8	30°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100.0

113.49

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100.0

0.0

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100.0

0.0

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100.0

65.0

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Isopropanol

30 % v/v

100.0

3.41

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100.0

30.48

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100.0

89.63

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethylene Glycol

30 % v/v

100.0

125.06

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethylene Glycol

20 % v/v

100.0

94.01

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethylene Glycol

10 % v/v

100.0

92.48

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

50.02

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100.0

75.15

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetone

10 % v/v

100.0

55.52

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

30 % v/v

100.0

19.94

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

20 % v/v

100.0

107.09

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

10 % v/v

100.0

95.35

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

30 % v/v

100.0

74.11

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

20 % v/v

100.0

111.01

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

10 % v/v

100.0

101.16

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

1-Butanol

30 % v/v

100.0

0.91

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

1-Butanol

20 % v/v

100.0

1.88

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

1-Butanol

10 % v/v

100.0

11.04

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetone

30 % v/v

100.0

2.55

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetone

20 % v/v

100.0

18.24

Direct

Continuous

50 mM phosphate-citrate buffer

30°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in %)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Esterase from Shewanella sp.

Enzyme Description

Sequence

Experimental Data (24 measurements)

Visualization : Activity — Classical

Structure

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