Esterase (gene EstDZ2) from a Kamtchatka hot spring metagenomic library

Enzyme Description

Species
Na (Kamtchatka hot spring metagenomic library)
Extremophile
Yes "thermophilic" organism
EC Number
3.1.1.1 β†—

Sequence

Length: 271 amino acids
MRTILYATLAVTLMSVSLSSAQPAPPFSFQVYTDVVYYDGPDAHPTKHRLDLFVPDGLQDAPVLIFVHGGGWTSGDKNLYAFIGQAFAAQGFATAVINYRLSPQVQHPAHIEDVARAFSWVYKNIARYGGNPEKIFVTGHSAGGHLTALLALDEKYLQAHGLTLAAIKGAIPISGVYDVTPVLALYRTVFGADPDQRWDASPLAHVGPNKPPFLILYAQFDFPGLGLQAQQLFAELRTHNNEATLTEIPNRDHVTIVATIGASNDPTTEEI
Dimitra Zarafeta et al. (2016) β€” Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases
Scientific Reports  Β· doi:10.1038/srep38886 β†—  Β· Activity - Classical Stability - Incubation
48 measurements ▼
Database ID
UniProt: A0A1L2DXZ2 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (48 measurements)

48 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 30 % v/v 100 105 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 10 % v/v 100 110 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 50 % v/v 100 0 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 30 % v/v 100 25 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 10 % v/v 100 40 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isooctane 50 % v/v 100 0 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isooctane 30 % v/v 100 25 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isooctane 10 % v/v 100 52 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 50 % v/v 100 0 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100 22 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 113 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Butanol 50 % v/v 100 0 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Butanol 30 % v/v 100 2 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isopropanol 50 % v/v 100 0 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Butanol 10 % v/v 100 18 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 50 % v/v 100 23 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 10 % v/v 100 109 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 30 % v/v 100 93 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 50 % v/v 100 6 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 10 % v/v 100 117 % Digital Continuous 25 mM Tris-HCl, 0.05% Triton X-100 8 50Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, "reaction under constant agitation" Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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